STAT3 antibody (pSer727)

Catalog No. ABIN6284752

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: AA 670-750, pSer727

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Stat3 is a protein encoded by the STAT3 gene which is approximately 88 kDa. Stat3 is localised to the cytoplasm and nucleus. It is involved in the immune response IL-23 signalling pathway, RET signalling, Th17 cell differentiation and prolactin signalling pathway. This protein falls under the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. Stat3 is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in the STAT3 gene may result in Hyperimmunoglobulin E recurrent infection syndrome. STJ90410 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Stat3 protein which only binds about S727 when S727 is phosphorylated.

Specificity: Phospho-Stat3 (S727) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at S727.

Characteristics: Rabbit polyclonal to Phospho-Stat3 (S727).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Stat3 around the phosphorylation site of S727.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IP 1:200-500
ELISA 1:10000

Comment:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: Stat3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Molecular Weight: 80 kDa

Gene ID: 6774

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor