FOXO3 antibody (pSer253)

Catalog No. ABIN6287889

Product Details anti-FOXO3 Antibody

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Binding Specificity: AA 190-270, pSer253

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: FoxO3A is a protein encoded by the FOXO3 gene which is approximately 71,2 kDa. FoxO3A is localised to the cytoplasm and nucleus. It is involved in RET signalling, GAB1 signalosome, glioma and common cytokine receptor gamma-chain family signalling pathways. It is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. It also participates in post-transcriptional regulation of MYC. FoxO3A is expressed ubiquitously. Mutations in the FOXO3 gene may result in secondary acute leukemia. STJ90275 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous FoxO3A protein which only binds about S253 when S253 is phosphorylated.

Specificity: Phospho-FoxO3A (S253) Polyclonal Antibody detects endogenous levels of FoxO3A protein only when phosphorylated at S253.

Characteristics: Rabbit polyclonal to Phospho-FoxO3A (S253).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human FoxO3A around the phosphorylation site of S253.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000

Comment:

Ubiquitous.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FoxO3A (FOXO3 Products)

Synonyms: AF6q21 antibody, FKHRL1 antibody, FKHRL1P2 antibody, FOXO2 antibody, FOXO3A antibody, zgc:92176 antibody, wu:fb50g02 antibody, wu:fi33f07 antibody, Fkhrl1 antibody, Foxo3a antibody, FoxO3A antibody, 1110048B16Rik antibody, 2010203A17Rik antibody, C76856 antibody, Fkhr2 antibody, FOXO-3 antibody, FOXO3 antibody, foxo3a antibody, xfoxo3 antibody, foxO3 antibody, foxO antibody, fkhr antibody, foxo5 antibody, wu:fj65a12 antibody, zFkhr antibody, forkhead box O3 antibody, forkhead box O3A antibody, forkhead box O3 L homeolog antibody, forkhead box O3b antibody, FOXO3 antibody, foxo3a antibody, Foxo3 antibody, foxo3.L antibody, foxo3 antibody, foxO3 antibody, foxo3b antibody

Molecular Weight: 71 kDa

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis