STAT3 antibody (Internal Region, pTyr705)

Catalog No. ABIN964927

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: Internal Region, pTyr705

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: Anti-Stat3 antibodyis an affinity-purified antibody and is directed against the phosphorylatedform of human Stat3 protein at the Y705. Anti-Stat3 antibody was affinitypurified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizingpeptide. The resultant affinity purified antibody was then cross adsorbedagainst the non-phosphorylated form of the immunizing peptide. Anti-Stat3antibody is specific for the phosphorylated form of theprotein. Reactivity with non-phosphorylated Stat3 protein is minimal byELISA and western blot. A BLAST analysis was used to suggest crossreactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues fromother sources is not known.

Characteristics: Signal transducerand activator of transcription 3 (Stat3) belongs to a family of cytoplasmictranscription factors that can be activated by phophorylation by its cell surfacereceptor. Stat3 plays a key role in many cellular processes such as cell growthand apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses toactivated FGFR1, FGFR2, FGFR3 and FGFR4.Stat3 forms a homodimer or aheterodimer with a related family member (e.g. STAT1). Activation occursthrough phosphorylation of tyrosine 705and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerizationand nuclear translocation. Serine phosphorylation is important for stableDNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 canhave a dual role in cancer, it has been found that Stat3 protein can promoteoncogenesis and have a tumor suppressor role depending upon the mutationalbackground of the tumor.

Purification: purified

Sterility: Sterile filtered

Immunogen: This affinitypurified Stat3 antibody was prepared by repeated immunizations with aphosphorylated synthetic peptide corresponding to the region of amino acids surroundingtyrosine 705 of human Stat3.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: STAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: STAT3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Background: Signal transducer and activator of transcription 3 (Stat3) belongs to a family of cytoplasmic transcription factors that can be activated by phophorylation by its cell surfacereceptor. Stat3 plays a key role in many cellular processes such as cell growth and apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Stat3 forms a homodimer or a heterodimer with a related family member (e.g. STAT1). Activation occurs through phosphorylation of tyrosine 705 and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerization and nuclear translocation. Serine phosphorylation is important for stable DNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 can have a dual role in cancer, it has been found that Stat3 protein can promote oncogenesis and have a tumor suppressor role depending upon the mutational background of the tumor.
Synonyms: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 (phospho Y705), STAT3 (pTry705)

Gene ID: 6774

NCBI Accession: NP_644805

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor