anti-Rat (Rattus) Caspase 4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Caspase 4 Antibody (supplied by: Log in to see )

Antigen
Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies
  • ICE(rel)II
  • ICEREL-II
  • ICH-2
  • Mih1/TX
  • TX
  • Casp11
  • CASP13
  • CASP1
  • CASP4
  • Caspl
  • ich-3
  • Casp4
  • caspase 4
  • caspase 4, apoptosis-related cysteine peptidase
  • caspase-4
  • CASP4
  • Casp4
  • LOC100731032
Reactivity
Human, Mouse (Murine), Rat (Rattus)
197
111
50
21
3
1
1
Host
Rabbit
206
28
21
17
7
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
19
16
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
256
93
92
57
31
25
19
18
17
13
10
3
2
2
1
Supplier
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Catalog No. ABIN498677
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.58104 ABIN872827 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2893038 IHC IHC (p) WB Rabbit Glu124 Log in to see Polyclonal 0
1 ABIN2617985 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN886623 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN886629 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(197), (111), (50), (21), (3), (1), (1)
Host Rabbit
(206), (28), (21), (17), (7)
Clonality
Polyclonal
Conjugate This Caspase 4 antibody is un-conjugated
(19), (16), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(256), (93), (92), (57), (31), (25), (19), (18), (17), (13), (10), (3), (2), (2), (1)
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Product Details anti-Caspase 4 Antibody

Target Details Caspase 4 Application Details Handling Images
Specificity This antibody detects endogenous levels of Caspase-4 protein. (region surrounding Glu124)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE
Plasmids, Primers & others

Target Details Caspase 4

Product Details anti-Caspase 4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-4 (CASP4 Antibody Abstract)
Background A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/caspase-1, is comprised of caspase- 1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, and caspase-10. Ced- 3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the 112 kDa nuclear protein PARP into an 85 kDa apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX
Molecular Weight approx. 34 kDa
Gene ID 837
NCBI Accession NP_001216
UniProt P49662
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (ABIN498677) Immunohistochemistry (IHC) analyzes of Caspase-4 antibody in paraffin-embedded...
Western Blotting (WB) image for anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (ABIN498677) Western blot (WB) analyzes of Caspase-4 antibody in extracts from K562 cells.