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|Antigen||Teratocarcinoma-Derived Growth Factor 1 (TDGF1) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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|Specificity||This antibody is specific for Cripto-1 protein. Expect cross-reactivity with Cripto-3 (TDGF2) based on very high levels of sequence conservation.|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Characteristics||Concentration: 0.80 mg/mL (by UV absorbance at 280 nm)|
|Immunogen||Synthetic peptide corresponding to an internal sequence of human Cripto-1 protein|
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|Alternative Name||TDGF1 / CRIPTO (TDGF1 Antibody Abstract)|
|Background||Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1|
|Pathways||EGFR Signaling Pathway|
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ELISA: 1/50,000 - 1/150,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|