anti-Human LAT2 antibody for Immunofluorescence

Recommended LAT2 Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells Family, Member 2 (LAT2) Antibodies
  • LAB
  • NTAL
  • WBSCR15
  • WBSCR5
  • WSCR5
  • LAT2
  • MGC139435
  • Ntal
  • Wbscr5
  • AW125574
  • Wbscr15
  • linker for activation of T-cells family member 2
  • linker for activation of T cells family, member 2
  • LAT2
  • Lat2
Epitope
AA 91-244
16
15
11
11
11
10
8
8
6
5
5
5
4
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
117
35
24
3
3
3
1
1
1
1
1
Host
Mouse
95
35
14
1
Clonality
Monoclonal
Conjugate
This LAT2 antibody is un-conjugated
8
6
6
3
3
3
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
134
43
31
29
20
16
12
6
4
3
3
2
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN120727
$ 313.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
5.5 ABIN536095 FACS IF IP WB Mouse IgG1 Log in to see NAP-07 3
4 ABIN532824 FACS IF IP WB Mouse IgG1 Log in to see NAP-07 0
-3.6835604 ABIN268974 CyTOF FACS ICC IF IP WB Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN5946787 FACS ICC IF Janelia Fluor® 549 Mouse IgG1 AA 91-244 Log in to see NAP-07 0
-11.159155 ABIN5946788 FACS ICC IF Janelia Fluor® 646 Mouse IgG1 AA 91-244 Log in to see NAP-07 0
-11.159155 ABIN4340815 FACS ICC IF WB DyLight 755 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340816 FACS ICC IF WB DyLight 680 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340817 FACS ICC IF WB DyLight 488 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340826 FACS ICC IF WB Alexa Fluor 405 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340827 FACS ICC IF WB Alexa Fluor 488 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340828 FACS ICC IF WB Alexa Fluor 647 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340829 FACS ICC IF WB Alexa Fluor 700 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340811 FACS ICC IF WB DyLight 350 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340812 FACS ICC IF WB DyLight 405 Mouse IgG1 Log in to see NAP-07 0
-11.159155 ABIN4340813 FACS ICC IF IP WB PerCP Mouse IgG1 Log in to see NAP-07 0

General

Antigen Linker For Activation of T Cells Family, Member 2 (LAT2) Antibodies
Epitope AA 91-244
(16), (15), (11), (11), (11), (10), (8), (8), (6), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(117), (35), (24), (3), (3), (3), (1), (1), (1), (1), (1)
Host Mouse
(95), (35), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT2 antibody is un-conjugated
(8), (6), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(134), (43), (31), (29), (20), (16), (12), (6), (4), (3), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-LAT2 Antibody

Target Details LAT2 Application Details Handling References for anti-LAT2 antibody (ABIN120727) Images
Specificity The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Purification Protein A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Recombinant cytoplasmic domain (aa 91-244) of human NTAL
Clone NAP-07
Isotype IgG1
Plasmids, Primers & others

Target Details LAT2

Product Details anti-LAT2 Antibody Application Details Handling References for anti-LAT2 antibody (ABIN120727) Images back to top
Antigen
Alternative Name LAT2 / WBSCR15 (LAT2 Antibody Abstract)
Background NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
Gene ID 7462
UniProt Q9GZY6
Pathways Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT2 Antibody Target Details LAT2 Handling References for anti-LAT2 antibody (ABIN120727) Images back to top
Application Notes Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details References for anti-LAT2 antibody (ABIN120727) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months

References for anti-LAT2 antibody (ABIN120727)

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details Handling Images back to top
Product cited in:

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, 2004

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Background publications

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002