anti-Rat (Rattus) LAT2 antibody for Immunofluorescence

Recommended LAT2 Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells Family, Member 2 (LAT2) Antibodies
  • LAB
  • NTAL
  • WBSCR15
  • WBSCR5
  • WSCR5
  • LAT2
  • MGC139435
  • Ntal
  • Wbscr5
  • AW125574
  • Wbscr15
  • linker for activation of T-cells family member 2
  • linker for activation of T cells family, member 2
  • LAT2
  • Lat2
Epitope
AA 30-203
16
15
11
11
11
10
8
8
6
5
5
5
4
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
118
35
23
3
3
3
1
1
1
1
1
Host
Rabbit
94
36
14
1
Clonality
Polyclonal
Conjugate
This LAT2 antibody is un-conjugated
8
6
6
3
3
3
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
134
43
32
29
21
16
12
6
4
3
3
2
1
1
Options
Supplier
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Available images

Catalog No. ABIN238345
$ 313.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
-8.123236 ABIN4340809 ICC IF WB Rabbit AA 30-203 Log in to see Polyclonal 0

General

Antigen Linker For Activation of T Cells Family, Member 2 (LAT2) Antibodies
Epitope AA 30-203
(16), (15), (11), (11), (11), (10), (8), (8), (6), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(118), (35), (23), (3), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(94), (36), (14), (1)
Clonality
Polyclonal
Conjugate This LAT2 antibody is un-conjugated
(8), (6), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(134), (43), (32), (29), (21), (16), (12), (6), (4), (3), (3), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT2 Antibody

Target Details LAT2 Application Details Handling References for anti-LAT2 antibody (ABIN238345) Images
Specificity This polyclonal antibody reacts with Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 25 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
Purification Protein-A Affinity Chromatography
Immunogen Recombinant Mouse NTAL (aa 30-203).
Plasmids, Primers & others

Target Details LAT2

Product Details anti-LAT2 Antibody Application Details Handling References for anti-LAT2 antibody (ABIN238345) Images back to top
Antigen
Alternative Name LAT2 / WBSCR15 (LAT2 Antibody Abstract)
Background NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
Gene ID 56743
NCBI Accession NP_064428
UniProt Q9JHL0
Pathways Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT2 Antibody Target Details LAT2 Handling References for anti-LAT2 antibody (ABIN238345) Images back to top
Application Notes Western Blotting (Non-reducing conditions): Recommended dilution: 1 μg/mLPositive control: mouse spleen. Sample preparation: Mix lysate with non-reducing SDS-PAGE sample buffer. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details References for anti-LAT2 antibody (ABIN238345) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-LAT2 antibody (ABIN238345)

Product Details anti-LAT2 Antibody Target Details LAT2 Application Details Handling Images back to top
Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Images

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Supplier Images
 image for anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-203) antibody (ABIN238345) anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-203) antibody
 image for anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-203) antibody (ABIN238345) anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-203) antibody (Image 2)