anti-Human JAK3 antibody for Immunohistochemistry

Recommended JAK3 Antibody (supplied by: Log in to see )

Antigen
Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
  • JAK-3
  • JAK3_HUMAN
  • JAKL
  • L-JAK
  • LJAK
  • fae
  • wil
  • RATJAK3
  • JAK
  • Janus kinase 3
  • JAK3
  • Jak3
Epitope
pTyr981
19
18
15
15
10
8
7
7
7
5
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
116
70
53
2
2
2
2
2
1
Host
Rabbit
88
34
Clonality
Polyclonal
Conjugate
This JAK3 antibody is un-conjugated
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
72
50
27
25
18
14
10
9
8
7
1
1
1
Supplier
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Catalog No. ABIN6271486
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.970049 ABIN6271484 ELISA ICC IF IHC WB Rabbit IgG pTyr904 Log in to see Polyclonal 0
15.579589 ABIN3182638 ELISA IHC WB Rabbit IgG pTyr785 Log in to see Polyclonal 0
15.579589 ABIN3185259 ELISA IF IHC WB Rabbit IgG Ser645 Log in to see Polyclonal 0
12.970049 ABIN1532701 IF IHC ELISA WB Rabbit IgG AA 751-800 Log in to see Polyclonal 1
12.579589 ABIN5618125 ELISA IHC WB Rabbit IgG pTyr785 Log in to see Polyclonal 0
12.579589 ABIN5618126 ELISA IF IHC WB Rabbit IgG Tyr785 Log in to see Polyclonal 0
9.970049 ABIN1531677 IHC ELISA WB Rabbit IgG AA 751-800, pTyr785 Log in to see Polyclonal 0
7 ABIN1859526 ICC IHC WB Rabbit IgG AA 716-967 Log in to see Polyclonal 0
7 ABIN2936002 IF/ICC IHC IP WB Rabbit AA 716-967 Log in to see Polyclonal 0
4 ABIN1577387 IF IHC ELISA WB Rabbit IgG Tyr785 Log in to see Polyclonal 0
4 ABIN1577385 IHC ELISA WB Rabbit IgG pTyr785 Log in to see Polyclonal 0
1 ABIN2755677 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
1 ABIN3066105 IF/ICC IHC WB Rabbit Center Log in to see Polyclonal 0
1 ABIN6105001 ELISA IHC WB Rabbit IgG pTyr785 Log in to see Polyclonal 0
1 ABIN5902437 ELISA IHC WB Rabbit IgG Tyr785 Log in to see Polyclonal 0
1 ABIN6105312 ELISA IF/ICC IHC WB Rabbit IgG Tyr785 Log in to see Polyclonal 0
1 ABIN6024602 IF/ICC IHC IP WB Mouse Log in to see 0
1 ABIN5558352 ELISA IHC IP WB FITC Rabbit phosphorylated Log in to see Polyclonal 0
1 ABIN5558351 ELISA IHC IP WB Biotin Rabbit phosphorylated Log in to see Polyclonal 0
1 ABIN5558350 ELISA IHC IP WB Rabbit phosphorylated Log in to see Polyclonal 0

General

Antigen Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
Epitope pTyr981
(19), (18), (15), (15), (10), (8), (7), (7), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(116), (70), (53), (2), (2), (2), (2), (2), (1)
Host Rabbit
(88), (34)
Clonality
Polyclonal
Conjugate This JAK3 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(72), (50), (27), (25), (18), (14), (10), (9), (8), (7), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-JAK3 Antibody

Target Details JAK3 Application Details Handling References for anti-JAK3 antibody (ABIN6271486) Images
Specificity JAK3 (Phospho-Tyr981) Antibody detects endogenous levels of JAK3 only when phosphorylated at Tyr981
Cross-Reactivity Human, Mouse (Murine)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human JAK3 (Phospho-Tyr981)
Isotype IgG
Plasmids, Primers & others

Target Details JAK3

Product Details anti-JAK3 Antibody Application Details Handling References for anti-JAK3 antibody (ABIN6271486) Images back to top
Antigen
Alternative Name JAK3 (JAK3 Antibody Abstract)
Background

Description: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 Molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Gene: JAK3

Molecular Weight 125kDa
Gene ID 3718
UniProt P52333
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded protein response

Application Details

Product Details anti-JAK3 Antibody Target Details JAK3 Handling References for anti-JAK3 antibody (ABIN6271486) Images back to top
Application Notes WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details References for anti-JAK3 antibody (ABIN6271486) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

References for anti-JAK3 antibody (ABIN6271486)

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling Images back to top
Product cited in:

Chen, Zhou, Liu, Huang, Liu, Kang, Chen, Guo, Zhu, Sun: "Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway." in: Oncology reports, Vol. 39, Issue 3, pp. 1081-1089, 2018

Images

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling References for anti-JAK3 antibody (ABIN6271486) back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) (pTyr981) antibody (ABIN6271486) ABIN6271487 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeab...
Immunohistochemistry (IHC) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) (pTyr981) antibody (ABIN6271486) ABIN6271487 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) (pTyr981) antibody (ABIN6271486) ABIN6271487 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) (pTyr981) antibody (ABIN6271486) ABIN6271487 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was for...
Western Blotting (WB) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) (pTyr981) antibody (ABIN6271486) Western blot analysis of JAK3 (Phospho-Tyr981) using UV treated COS7 whole cell lysates