anti-Mouse (Murine) JAK3 antibody for Western Blotting

Recommended JAK3 Antibody (supplied by: Log in to see )

Antigen
Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
  • JAK-3
  • JAK3_HUMAN
  • JAKL
  • L-JAK
  • LJAK
  • fae
  • wil
  • RATJAK3
  • JAK
  • Janus kinase 3
  • JAK3
  • Jak3
Reactivity
Human, Mouse (Murine)
116
70
53
2
2
2
2
2
1
Host
Mouse
89
33
Clonality
Monoclonal
Conjugate
This JAK3 antibody is un-conjugated
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
72
50
27
26
17
15
10
9
8
7
1
1
1
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Catalog No. ABIN969224
$ 345.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.302324 ABIN6271486 ELISA ICC IF IHC WB Rabbit IgG pTyr981 Log in to see Polyclonal 1
19.802324 ABIN1844119 ELISA FACS IF/ICC WB Mouse IgG1 Log in to see 0
16.802324 ABIN2869455 ELISA FACS ICC WB Mouse IgG1 Log in to see 5H2 3
16.802324 ABIN6271484 ELISA ICC IF IHC WB Rabbit IgG pTyr904 Log in to see Polyclonal 0
16.562979 ABIN3182638 ELISA IHC WB Rabbit IgG pTyr785 Log in to see Polyclonal 0
16.562979 ABIN3185259 ELISA IF IHC WB Rabbit IgG Ser645 Log in to see Polyclonal 0
16.562979 ABIN634320 WB Rabbit Log in to see Polyclonal 0
13.802324 ABIN1109428 EIA IF WB Mouse IgG1 Log in to see 5H2 3
12.760654 ABIN4901830 ELISA FACS IF WB Mouse IgG1 Log in to see 5H2 0
12.302324 ABIN658182 WB Rabbit Ig Fraction AA 133-161, N-Term Log in to see Polyclonal 1
11.5 ABIN681013 IF (p) IHC (p) WB Rabbit IgG AA 610-660 Log in to see Polyclonal 1
10.802324 ABIN1532701 IF IHC ELISA WB Rabbit IgG AA 751-800 Log in to see Polyclonal 1
10.802324 ABIN1577384 FACS IF ELISA WB Mouse Log in to see 0
7.8023243 ABIN1531677 IHC ELISA WB Rabbit IgG AA 751-800, pTyr785 Log in to see Polyclonal 0
7.8023243 ABIN498150 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
7.8023243 ABIN1859525 ICC IHC WB Rabbit IgG AA 517-749 Log in to see Polyclonal 0
7.8023243 ABIN2997854 WB Rabbit IgG Log in to see Polyclonal 0
7.8023243 ABIN2936003 IF/ICC IHC IP WB Rabbit AA 517-749 Log in to see Polyclonal 0
7 ABIN742883 IF (p) IHC (p) WB Rabbit IgG pTyr980, pTyr981, AA 971-990 Log in to see Polyclonal 0
7 ABIN5534437 WB Rabbit Ig Fraction AA 133-161, N-Term Log in to see Polyclonal 0

General

Antigen Tyrosine-Protein Kinase JAK3 (JAK3) Antibodies
Reactivity Human, Mouse (Murine)
(116), (70), (53), (2), (2), (2), (2), (2), (1)
Host Mouse
(89), (33)
Clonality (Clone)
Monoclonal   ( )
Conjugate This JAK3 antibody is un-conjugated
(5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(72), (50), (27), (26), (17), (15), (10), (9), (8), (7), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-JAK3 Antibody

Target Details JAK3 Application Details Handling References for anti-JAK3 antibody (ABIN969224) Images
Purification Ascites
Immunogen Purified recombinant fragment of human JAK3 expressed in E. Coli.
Clone 5H2
Isotype IgG1
Plasmids, Primers & others

Target Details JAK3

Product Details anti-JAK3 Antibody Application Details Handling References for anti-JAK3 antibody (ABIN969224) Images back to top
Antigen
Alternative Name JAK3 (JAK3 Antibody Abstract)
Background JAK3, Janus kinase 3. It is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).
Molecular Weight 125 kDa
Gene ID 3718
HGNC 3718
Pathways JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded protein response

Application Details

Product Details anti-JAK3 Antibody Target Details JAK3 Handling References for anti-JAK3 antibody (ABIN969224) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details References for anti-JAK3 antibody (ABIN969224) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-JAK3 antibody (ABIN969224)

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling Images back to top
Product cited in:

Wood, Goodman, Uckun: "Jak3 expression and genomic sequence in pediatric acute lymphoblastic leukemia." in: Leukemia & lymphoma, Vol. 43, Issue 12, pp. 2355-62, 2003

Background publications

Lai, Jin, Graham, Witthuhn, Ihle, Liu: "A kinase-deficient splice variant of the human JAK3 is expressed in hematopoietic and epithelial cancer cells." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25028-36, 1995

Kawamura, McVicar, Johnston, Blake, Chen, Lal, Lloyd, Kelvin, Staples, Ortaldo: "Molecular cloning of L-JAK, a Janus family protein-tyrosine kinase expressed in natural killer cells and activated leukocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 14, pp. 6374-8, 1994

Images

Product Details anti-JAK3 Antibody Target Details JAK3 Application Details Handling References for anti-JAK3 antibody (ABIN969224) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) antibody (ABIN969224) Figure 1: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and nega...
Immunocytochemistry (ICC) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) antibody (ABIN969224) Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells...
Immunocytochemistry (ICC) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) antibody (ABIN969224) Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (...
Western Blotting (WB) image for anti-Tyrosine-Protein Kinase JAK3 (JAK3) antibody (ABIN969224) Figure 4: Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1) .