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|Antigen||Protein Inhibitor of Activated STAT, 2 (PIAS2) Antibodies|
|Conjugate||This PIAS2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PIAS2 AntibodyTarget Details PIAS2 Application Details Handling References for anti-PIAS2 antibody (ABIN356767) Images
|Specificity||This antibody is specific to PIAS2 (PIASx1/2) (C-term).|
|Purification||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIASx1/2.|
Target Details PIAS2Product Details anti-PIAS2 Antibody Application Details Handling References for anti-PIAS2 antibody (ABIN356767) Images back to top
|Alternative Name||PIAS2 (PIAS2 Antibody Abstract)|
|Background||PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.Synonyms: ARIP3, Androgen receptor-interacting protein 3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, Miz1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2|
|Gene ID||9063, 9606|
|Pathways||JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling|
Application DetailsProduct Details anti-PIAS2 Antibody Target Details PIAS2 Handling References for anti-PIAS2 antibody (ABIN356767) Images back to top
ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIAS2 Antibody Target Details PIAS2 Application Details References for anti-PIAS2 antibody (ABIN356767) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PIAS2 antibody (ABIN356767)Product Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling Images back to top
Sakurai, Itoh, Higashitsuji, Nagao, Nonoguchi, Chiba, Fujita: "A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells." in: The Journal of biological chemistry, Vol. 279, Issue 15, pp. 15505-14, 2004
Arora, Liu, He, Kim, Murphy, Murphy, Modlin, Shuai: "PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21327-30, 2003
Wu, Cetinkaya, Munoz-Alonso, von der Lehr, Bahram, Beuger, Eilers, Leon, Larsson: "Myc represses differentiation-induced p21CIP1 expression via Miz-1-dependent interaction with the p21 core promoter." in: Oncogene, Vol. 22, Issue 3, pp. 351-60, 2003
Liu, Liao, Rao, Kushner, Chung, Chang, Shuai: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998
Wu, Wu, Ma, Sangiorgi, Wu, Bell, Lyons, Maxson: "Miz1, a novel zinc finger transcription factor that interacts with Msx2 and enhances its affinity for DNA." in: Mechanisms of development, Vol. 65, Issue 1-2, pp. 3-17, 1997
ImagesProduct Details anti-PIAS2 Antibody Target Details PIAS2 Application Details Handling References for anti-PIAS2 antibody (ABIN356767) back to top