anti-Human STAT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended STAT1 Antibody

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • stat1
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • signal transducer and activator of transcription 1a
  • STAT1
  • Stat1
  • stat1.L
  • stat4
  • stat1a
Epitope
AA 721-733
101
74
52
17
17
15
9
8
8
8
8
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
564
313
282
33
29
26
22
22
16
13
11
7
7
5
2
2
2
1
1
1
1
1
Host
Mouse
485
105
2
2
1
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is un-conjugated
18
14
13
11
11
11
11
11
11
6
6
5
5
5
5
5
4
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
448
205
163
112
90
65
48
19
18
9
7
6
5
3
1
1
Options

Available images

Catalog No. ABIN94471
$ 428.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19 ABIN1501168 IF IHC IHC (p) WB Mouse IgG1 15H3 0
19 ABIN1501169 IF IHC IHC (p) WB Mouse IgG1 4H9 0
17.5 ABIN3043938 IHC (p) WB Rabbit IgG AA 114-143, N-Term Polyclonal 3
14.786058 ABIN214518 ICC IHC IHC (p) IP WB Rabbit IgG C-Term Polyclonal 0
14.786058 ABIN5611535 EIA IHC (p) WB Rabbit IgG C-Term Polyclonal 0
14.786058 ABIN5611519 EIA IHC (p) WB Rabbit IgG pSer727 Polyclonal 0
14.786058 ABIN1386191 IF (p) IHC (p) WB Rabbit IgG Polyclonal 0
13.551713 ABIN4902308 IHC (p) Mouse IgG1 AA 640-720 8H11 0
13.551713 ABIN4902309 IHC (p) Mouse IgG1 AA 640-720 12E8 0
13.551713 ABIN4902310 IHC (p) Mouse IgG1 AA 640-720 5H7 0
13.551713 ABIN1689931 IHC (p) WB Rabbit Polyclonal 0
13.286058 ABIN674889 IF (p) IHC (p) WB Rabbit IgG Polyclonal 3
13.286058 ABIN732623 IF (p) IHC (p) WB Rabbit IgG pTyr701 Polyclonal 1
13 ABIN1501170 IHC IHC (p) WB Mouse IgG1 13B10 0
11.786058 ABIN239822 IHC IHC (p) IP WB Mouse IgG2b SM1 0
11.786058 ABIN498804 IHC (p) WB Rabbit pSer727 Polyclonal 0
11.786058 ABIN498895 IHC (p) WB Rabbit Polyclonal 0
11.5 ABIN5518871 IHC (p) WB Rabbit IgG AA 2-230 Polyclonal 2
11.5 ABIN537760 IHC (p) ELISA WB Rabbit pSer727 Polyclonal 3
10 ABIN6652217 IF IHC (p) WB Rabbit pSer727 Polyclonal 4

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope AA 721-733
(101), (74), (52), (17), (17), (15), (9), (8), (8), (8), (8), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(564), (313), (282), (33), (29), (26), (22), (22), (16), (13), (11), (7), (7), (5), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(485), (105), (2), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is un-conjugated
(18), (14), (13), (11), (11), (11), (11), (11), (11), (6), (6), (5), (5), (5), (5), (5), (4), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(448), (205), (163), (112), (90), (65), (48), (19), (18), (9), (7), (6), (5), (3), (1), (1)
Pubmed 5 references available
Supplier

Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN94471) Images
Specificity The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Cross-Reactivity (Details) Mouse, Human, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Clone SM1
Isotype IgG2b
Plasmids, Primers & others

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 antibody (ABIN94471) Images back to top
Antigen
Alternative Name STAT1 (STAT1 Antibody Abstract)
Background STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 antibody (ABIN94471) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 antibody (ABIN94471) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-STAT1 antibody (ABIN94471)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Background publications

Soond, Townsend, Barry, Knight, Latchman, Stephanou: "ERK and the F-box protein betaTRCP target STAT1 for degradation." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 16077-83, 2008

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000

Frank: "STAT signaling in the pathogenesis and treatment of cancer." in: Molecular medicine (Cambridge, Mass.), Vol. 5, Issue 7, pp. 432-56, 1999

Product cited in:

Kovarik, Boudny, Kocak, Lauerova, Fait, Vagundova: "Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation." in: International journal of molecular medicine, Vol. 12, Issue 3, pp. 335-40, 2003

Boudný, Kocák, Lauerová, Kovarík: "Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients." in: Folia biologica, Vol. 49, Issue 4, pp. 142-6, 2003

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN94471) back to top
Supplier Images
Western Blotting (WB) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 721-733) antibody (ABIN94471) Induction of phosphorylation of STAT1 (Western Blotting) Fig. 1: Induction of phospho...