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|Antigen||Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This STAT1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
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Product Details anti-STAT1 AntibodyTarget Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN968751) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Phosphorylated Human Stat1 (pY701) Peptide|
Target Details STAT1Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 antibody (ABIN968751) Images back to top
|Alternative Name||Stat1 (STAT1 Antibody Abstract)|
Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs).
The 14/P-STAT1monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.
|Molecular Weight||91/84 kDa|
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 antibody (ABIN968751) Images back to top
Related Products: ABIN968533, ABIN968534, ABIN967389, ABIN967758
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 antibody (ABIN968751) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-STAT1 antibody (ABIN968751)Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
|Product cited in:||
Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, 1997
Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993
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