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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies|
|Conjugate||This MAP3K1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|2 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K1 AntibodyTarget Details MAP3K1 Application Details Handling References for anti-MAP3K1 antibody (ABIN358289) Images
|Specificity||This antibody detects MAP3K1 pThr1383. Predicted to cross react with Mouse (100 % Antigen Homology).|
|Purification||Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1383 of human MAP3K1.|
Target Details MAP3K1Product Details anti-MAP3K1 Antibody Application Details Handling References for anti-MAP3K1 antibody (ABIN358289) Images back to top
|Alternative Name||MAP3K1 (MAP3K1 Antibody Abstract)|
|Background||Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.Synonyms: MAPK/ERK kinase kinase 1, MAPKKK1, MEKK, MEKK1, Mitogen-activated protein kinase kinase kinase 1|
|Molecular Weight||164340 Da|
|Gene ID||4214, 9606|
|Pathways||MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling References for anti-MAP3K1 antibody (ABIN358289) Images back to top
ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details References for anti-MAP3K1 antibody (ABIN358289) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP3K1 antibody (ABIN358289)Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling Images back to top
Mokhtari, Myers, Welsh: "MAPK kinase kinase-1 is essential for cytokine-induced c-Jun NH2-terminal kinase and nuclear factor-kappaB activation in human pancreatic islet cells." in: Diabetes, Vol. 57, Issue 7, pp. 1896-904, 2008
Dassé, Bridoux, Baranek, Lambert, Salesse, Sowa, Martiny, Trentesaux, Petitfrère: "Tissue inhibitor of metalloproteinase-1 promotes hematopoietic differentiation via caspase-3 upstream the MEKK1/MEK6/p38alpha pathway." in: Leukemia, Vol. 21, Issue 4, pp. 595-603, 2007
ImagesProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling References for anti-MAP3K1 antibody (ABIN358289) back to top