anti-Human MAP3K12 antibody for Western Blotting

Recommended MAP3K12 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) Antibodies
  • DLK
  • DLK-1
  • Delta1
  • FA1
  • PREF1
  • Pref-1
  • ZOG
  • pG2
  • MEKK12
  • MUK
  • ZPK
  • ZPKP1
  • PK
  • Zpk
  • zgc:77370
  • MGC151798
  • dlk
  • map3k12
  • map3k12a
  • mekk12
  • muk
  • zpk
  • zpkp1
  • DKFZp459K2433
  • map3k12-a
  • map3k12-b
  • map3k12b
  • delta like non-canonical Notch ligand 1
  • mitogen-activated protein kinase kinase kinase 12
  • mitogen activated protein kinase kinase kinase 12
  • mitogen-activated protein kinase kinase kinase 12 S homeolog
  • mitogen-activated protein kinase kinase kinase 12 L homeolog
  • DLK1
  • MAP3K12
  • Map3k12
  • map3k12
  • map3k12.S
  • map3k12.L
Epitope
AA 828-859, C-Term
17
9
4
3
2
1
1
1
Reactivity
Human, Mouse (Murine)
55
25
14
Host
Rabbit
46
12
Clonality
Polyclonal
Conjugate
This MAP3K12 antibody is un-conjugated
6
5
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
50
35
28
7
5
3
1
1
1
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Catalog No. ABIN391319
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.232922 ABIN4332644 IHC IHC (fro) IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 1
3.232922 ABIN359408 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
3.232922 ABIN1539909 WB Mouse IgG1 AA 828-859 Log in to see 473CT23-12-5 0
3.232922 ABIN2737134 WB Rabbit IgG Log in to see Polyclonal 0
3.232922 ABIN1539908 WB Mouse IgG1 AA 828-859 Log in to see 473CT23-12-5 0
3.232922 ABIN3031847 IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal 0
1 ABIN655162 IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 2
1 ABIN4332642 ELISA WB FITC Rabbit Log in to see Polyclonal 6
1 ABIN1497853 WB Mouse IgG1 AA 828-859 Log in to see 0
1 ABIN2306936 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1911680 IHC ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1911681 IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1911682 IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1911683 IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1911679 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5682801 ELISA IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2306932 IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2306933 ELISA WB Mouse IgG1 Log in to see 473CT23-12-5 0
1 ABIN2306934 IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2306930 IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) Antibodies
Epitope AA 828-859, C-Term
(17), (9), (4), (3), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(55), (25), (14)
Host Rabbit
(46), (12)
Clonality (Clone)
Polyclonal   ( )
Conjugate This MAP3K12 antibody is un-conjugated
(6), (5), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (35), (28), (7), (5), (3), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-MAP3K12 Antibody

Target Details MAP3K12 Application Details Handling References for anti-MAP3K12 antibody (ABIN391319) Images
Specificity This ZPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830~859 amino acids from the C-terminal region of human ZPK.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This ZPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828-859 AA from the C-terminal region of human ZPK.
Clone RB0976
Isotype Ig Fraction

Target Details MAP3K12

Product Details anti-MAP3K12 Antibody Application Details Handling References for anti-MAP3K12 antibody (ABIN391319) Images back to top
Antigen
Alternative Name ZPK (MAP3K12 Antibody Abstract)
Background ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.
Synonyms: Mitogen-activated protein kinase kinase kinase 12, Leucine-zipper protein kinase, MAP3K12, M3KC, Mixed lineage kinase, Dual leucine zipper bearing kinase, DLK, MAPK-upstream kinase, MUK
Molecular Weight 93188 DA
Gene ID 7786
UniProt Q12852
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Handling References for anti-MAP3K12 antibody (ABIN391319) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details References for anti-MAP3K12 antibody (ABIN391319) Images back to top
Format Liquid
Concentration 1.19 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-MAP3K12 antibody (ABIN391319)

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details Handling Images back to top
Product cited in:

Ye, Li, Yang, Xie, Yu, Ma, Zhu, Zhao, Lv: "MicroRNA-130a Targets MAP3K12 to Modulate Diabetic Endothelial Progenitor Cell Function." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 2, pp. 712-26, 2015

Couture, Blouin: "The DLK gene is a transcriptional target of PPAR?." in: The Biochemical journal, Vol. 438, Issue 1, pp. 93-101, 2011

Background publications

Itoh, Wang, Ito, Reddy, Itoh: "SP3 acts as a positive regulator on the core promoter of human ZPK gene." in: Biochemical and biophysical research communications, Vol. 313, Issue 3, pp. 612-8, 2003

Fukuyama, Yoshida, Yamashita, Deyama, Baba, Suzuki, Mohri, Ikezawa, Nakajima, Hirai, Ohno: "MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 28, pp. 21247-54, 2000

Reddy, Basu, Bannerman, Ikegaki, Reddy, Pleasure: "ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation." in: Oncogene, Vol. 18, Issue 31, pp. 4474-84, 1999

Mata, Merritt, Fan, Yu, Holzman: "Characterization of dual leucine zipper-bearing kinase, a mixed lineage kinase present in synaptic terminals whose phosphorylation state is regulated by membrane depolarization via calcineurin." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16888-96, 1996

Hirai, Izawa, Osada, Spyrou, Ohno: "Activation of the JNK pathway by distantly related protein kinases, MEKK and MUK." in: Oncogene, Vol. 12, Issue 3, pp. 641-50, 1996

Images

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details Handling References for anti-MAP3K12 antibody (ABIN391319) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) (AA 828-859), (C-Term) antibody (ABIN391319) Western blot analysis of anti-ZPK C-term Pab (ABIN391319) in HL-60 cell lysate (lane ...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) (AA 828-859), (C-Term) antibody (ABIN391319) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...