anti-Rat (Rattus) DPP4 antibody for Immunoprecipitation

Recommended DPP4 Antibody (supplied by: Log in to see )

Antigen
Dipeptidyl-Peptidase 4 (DPP4) Antibodies
  • ADABP
  • ADCP2
  • CD26
  • DPPIV
  • TP103
  • MGC81966
  • MOP9.8
  • MOP9_8
  • DPP4
  • si:ch73-2d23.3
  • Cd26
  • Dpp-4
  • THAM
  • dipeptidyl peptidase 4
  • dipeptidyl-peptidase 4 S homeolog
  • prolyl oligopeptidase family protein
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase 4
  • DPP4
  • dpp4.S
  • AT5G24260
  • dpp4
  • Dpp4
Reactivity
Rat (Rattus)
447
148
125
29
19
14
9
7
6
5
5
5
4
3
2
2
2
1
1
1
1
Host
Mouse
357
150
97
22
5
Clonality
Monoclonal
Conjugate
This DPP4 antibody is un-conjugated
61
59
44
29
16
16
13
13
11
10
9
9
9
9
9
6
6
5
5
5
5
5
4
4
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
418
152
124
105
95
64
46
38
16
13
13
10
8
7
4
4
4
3
2
1
1
Options
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Pubmed
8 references available
Catalog No. ABIN967645
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106155 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 5-00E-008 3
1 ABIN2479527 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-61 4
1 ABIN307872 FACS IHC (fro) IP WB Biotin Mouse IgG2a Log in to see OX-61 0
1 ABIN600758 IHC (fro) IP WB Mouse IgG1 Log in to see 0
1 ABIN600760 IHC (fro) FACS IP WB Mouse IgG2a Log in to see 0
1 ABIN600761 IHC (fro) IP WB Mouse IgG1 Log in to see 0
1 ABIN320309 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-61 1
1 ABIN4306069 FACS ICC IF IHC IP Mouse IgG2b kappa Log in to see 236-3 0
1 ABIN212849 FACS IP WB Mouse IgG2a Log in to see OX-61 0
1 ABIN1839610 IHC (fro) IP WB Mouse IgG1 Log in to see 5E8 0
1 ABIN1106158 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 5-00E-008 1
1 ABIN2224586 FACS IP IHC WB Mouse IgG2a Log in to see 3H1168 0
1 ABIN2224560 IP IHC WB Mouse IgG1 Log in to see 5E8 0
1 ABIN2224576 IP IHC WB Mouse IgG1 Log in to see 9G144 0
1 ABIN213164 FACS IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN5576791 ELISA IHC (fro) IP WB Mouse IgG1 Log in to see 5E8 0
1 ABIN161229 FACS IP IHC WB Biotin Mouse IgG2a Log in to see OX-61 0
1 ABIN161230 FACS IP IHC WB Biotin Mouse IgG2a Log in to see OX-61 0

General

Antigen Dipeptidyl-Peptidase 4 (DPP4) Antibodies
Reactivity Rat (Rattus)
(447), (148), (125), (29), (19), (14), (9), (7), (6), (5), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(357), (150), (97), (22), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DPP4 antibody is un-conjugated
(61), (59), (44), (29), (16), (16), (13), (13), (11), (10), (9), (9), (9), (9), (9), (6), (6), (5), (5), (5), (5), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(418), (152), (124), (105), (95), (64), (46), (38), (16), (13), (13), (10), (8), (7), (4), (4), (4), (3), (2), (1), (1)
Pubmed 8 references available
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Product Details anti-DPP4 Antibody

Target Details DPP4 Application Details Handling References for anti-DPP4 antibody (ABIN967645) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat dendritic cells enriched from thoracic duct lymph
Clone OX-61
Isotype IgG2a, kappa

Target Details DPP4

Product Details anti-DPP4 Antibody Application Details Handling References for anti-DPP4 antibody (ABIN967645) Images back to top
Antigen
Alternative Name CD26 (DPP4 Antibody Abstract)
Background The OX-61 antibody reacts with a type II transmembrane glycoprotein, CD26, which is a serine exoprotease identified as dipeptidyl/peptidase IV. Among various biological activities, rat CD26 binds fibronectin and collagen. Rat CD26 is involved in the costimulation of thymocyte proliferation in vitro, particularly the CD4-/CD8- subset, and is developmentally regulated on hematopoietic cells. Although mouse and human CD26 anchor ADA (adenosine deaminase) to cell membranes, rat CD26 does not function as an ADA-binding protein. Rat CD26 is expressed in lung endothelial cells, as well as in various epithelial cells. T cells express lower levels of CD26 than CD4-/CD8- thymocytes. The distribution of CD26 antigen in rat bone marrow cells is similar to that of human CD26. OX-61 monoclonal antibody stains CD4+, CD8+, and Ig+ lymphocytes, and the staining increases upon activation.
Pathways Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-DPP4 Antibody Target Details DPP4 Handling References for anti-DPP4 antibody (ABIN967645) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-DPP4 Antibody Target Details DPP4 Application Details References for anti-DPP4 antibody (ABIN967645) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-DPP4 antibody (ABIN967645)

Product Details anti-DPP4 Antibody Target Details DPP4 Application Details Handling Images back to top
Product cited in:

Iwaki-Egawa, Watanabe, Fujimoto: "CD26/dipeptidyl peptidase IV does not work as an adenosine deaminase-binding protein in rat cells." in: Cellular immunology, Vol. 178, Issue 2, pp. 180-6, 1997

Johnson, Zhu, Augustin-Voss, Pauli: "Lung endothelial dipeptidyl peptidase IV is an adhesion molecule for lung-metastatic rat breast and prostate carcinoma cells." in: The Journal of cell biology, Vol. 121, Issue 6, pp. 1423-32, 1993

Kameoka, Tanaka, Nojima, Schlossman, Morimoto: "Direct association of adenosine deaminase with a T cell activation antigen, CD26." in: Science (New York, N.Y.), Vol. 261, Issue 5120, pp. 466-9, 1993

Bristol, Sakaguchi, Appella, Doyle, Takács: "Thymocyte costimulating antigen is CD26 (dipeptidyl-peptidase IV). Costimulation of granulocyte, macrophage, and T lineage cell proliferation via CD26." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 367-72, 1992

Gorrell, Wickson, McCaughan: "Expression of the rat CD26 antigen (dipeptidyl peptidase IV) on subpopulations of rat lymphocytes." in: Cellular immunology, Vol. 134, Issue 1, pp. 205-15, 1991

McCaughan, Wickson, Creswick, Gorrell: "Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence." in: Hepatology (Baltimore, Md.), Vol. 11, Issue 4, pp. 534-44, 1990

Piazza, Callanan, Mowery, Hixson: "Evidence for a role of dipeptidyl peptidase IV in fibronectin-mediated interactions of hepatocytes with extracellular matrix." in: The Biochemical journal, Vol. 262, Issue 1, pp. 327-34, 1989

Hanski, Huhle, Gossrau, Reutter: "Direct evidence for the binding of rat liver DPP IV to collagen in vitro." in: Experimental cell research, Vol. 178, Issue 1, pp. 64-72, 1988