anti-Rat (Rattus) PDK4 antibody for ELISA

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
  • pyruvate dehydrogenase kinase 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pdk4
  • PDK4
  • Pdk4
Reactivity
Human, Mouse (Murine), Rat (Rattus)
146
76
18
2
2
2
1
1
1
1
1
Host
Rabbit
149
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
12
9
7
7
7
7
2
2
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
154
75
70
36
25
18
7
4
3
1
Supplier
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Catalog No. ABIN6266305
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN207477 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN5704127 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6042917 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6042902 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1091116 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2922765 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5930291 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1856862 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN4368833 IHC ELISA WB Rabbit Log in to see Polyclonal 0

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(146), (76), (18), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(149), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(12), (9), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(154), (75), (70), (36), (25), (18), (7), (4), (3), (1)
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling Images
Specificity PDK4 Antibody detects endogenous levels of total PDK4
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthetic peptide of humanPDK4
Isotype IgG
Plasmids, Primers & others

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background

Description: Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood  pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Gene: PDK4

Molecular Weight 46kDa
Gene ID 5166
UniProt Q16654
Pathways PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) antibody (ABIN6266305) Western blot analysis of extracts of mouse pancreas , using PDK4antibody. The lane on...
Immunohistochemistry (IHC) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) antibody (ABIN6266305) ABIN6277424 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehy...