MDM2 Protein (AA 1-491) (GST tag)
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- Target See all MDM2 Proteins
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-491
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This MDM2 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- MDM2 (Human) Recombinant Protein (P01)
- Sequence
- MCNTNMSVPT DGAVTTSQIP ASEQETLVRP KPLLLKLLKS VGAQKDTYTM KEVLFYLGQY IMTKRLYDEK QQHIVYCSND LLGDLFGVPS FSVKEHRKIY TMIYRNLVVV NQQESSDSGT SVSENRCHLE GGSDQKDLVQ ELQEEKPSSS HLVSRPSTSS RRRAISETEE NSDELSGERQ RKRHKSDSIS LSFDESLALC VIREICCERS SSSESTGTPS NPDLDAGVSE HSGDWLDQDS VSDQFSVEFE VESLDSEDYS LSEEGQELSD EDDEVYQVTV YQAGESDTDS FEEDPEISLA DYWKCTSCNE MNPPLPSHCN RCWALRENWL PEDKGKDKGE ISEKAKLENS TQAEEGFDVP DCKKTIVNDS RESCVEENDD KITQASQSQE SEDYSQPSTS SSIIYSSQED VKEFEREETQ DKEESVESSL PLNAIEPCVI CQGRPKNGCI VHGKTGHLMA CFTCAKKLKK RNKPCPVCRQ PIQMIVLTYF P
- Characteristics
- Human MDM2 full-length ORF ( ENSP00000258149, 1 a.a. - 491 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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LC-MS/MS-based targeted proteomics quantitatively detects the interaction between p53 and MDM2 in breast cancer." in: Journal of proteomics, Vol. 152, pp. 172-180, (2016) (PubMed).
: "
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LC-MS/MS-based targeted proteomics quantitatively detects the interaction between p53 and MDM2 in breast cancer." in: Journal of proteomics, Vol. 152, pp. 172-180, (2016) (PubMed).
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- Target
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- Alternative Name
- MDM2 (MDM2 Products)
- Synonyms
- ACTFS Protein, HDMX Protein, hdm2 Protein, zgc:109834 Protein, 1700007J15Rik Protein, AA415488 Protein, Mdm-2 Protein, MDM2 proto-oncogene Protein, MDM2 proto-oncogene S homeolog Protein, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) Protein, MDM2 oncogene, E3 ubiquitin protein ligase Protein, transformed mouse 3T3 cell double minute 2 Protein, MDM2 Protein, mdm2.S Protein, Mdm2 Protein, mdm2 Protein
- Background
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Full Gene Name: Mdm2 p53 binding protein homolog (mouse)
Synonyms: HDMX,MGC71221,hdm2 - Gene ID
- 4193
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
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