BAD antibody

Catalog No. ABIN126732

Product Details anti-BAD Antibody

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: This antibody specifically binds the BAD protein but does not recognize BAD/Bcl-2 heterodimers in Immunoprecipitation assays.

Characteristics: Synonyms: BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8, Bcl2 antagonist of cell death,Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter

Purification: Subsequent Thiopilic Adsorption and Size Exclusion Chromatography

Immunogen: Recombinant Human BAD.

Clone: 5-00E-006

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation: 1-2 μg/mL. Also suitable for Western blotting if cells overexpressing BAD are used (0.5 μg/mL forHRPO/ECL detection). Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Abstract: BAD Products

Synonyms: bad antibody, fa01b12 antibody, wu:fa01b12 antibody, wu:fa96d04 antibody, BAD antibody, BBC2 antibody, BCL2L8 antibody, AI325008 antibody, Bbc2 antibody, BCL2-associated agonist of cell death b antibody, BCL2 associated agonist of cell death antibody, BCL2-associated agonist of cell death antibody, badb antibody, BAD antibody, Bad antibody

Background: BAD is an important regulatory protein controlling cellsurvival/apoptosis. Unphosphorylated BAD heterodimerizes with Bcl-2 and Bcl-XL, thus neutralizing the anti-apoptotic properties of the Bcl proteins. Activation of different signal transduction pathways leads to phosphorylation of BAD at multiple serine residues, e.g. phosphorylation of serine 136 by PKB, serine 112 by MAPK, and serine 155 by PKA, and dissociation of the BAD/Bcl heterodimers.Synonyms: BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

Gene ID: 572

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events