Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This antibody is expected to recognize both reported isoforms (NP_000622.2 and NP_038202.1).
Purification
NCF4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
NCF4 antibody was raised against an 11 amino acid synthetic peptide near the internal region of NCF4.
NCF4
Reactivity: Human
WB, IHC, FACS
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 38 kDa band observed in lysates of Daudi lysate (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approximately 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.