NCF4 antibody (Internal Region)

Catalog No. ABIN2465987

Product Details anti-NCF4 Antibody

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: This antibody is expected to recognize both reported isoforms (NP_000622.2 and NP_038202.1).

Purification: NCF4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NCF4 antibody was raised against an 11 amino acid synthetic peptide near the internal region of NCF4.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 38 kDa band observed in lysates of Daudi lysate (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approximately 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: NCF4 (NCF4 Products)

Synonyms: p40phox antibody, AI451400 antibody, NCF antibody, P40PHOX antibody, SH3PXD4 antibody, neutrophil cytosolic factor 4 antibody, ncf4 antibody, Ncf4 antibody, NCF4 antibody

Gene ID: 4689

NCBI Accession: NP_000622, NP_038202

UniProt: Q15080