Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
Purification
Affinity chromatography purified
Immunogen
C-myc antibody (HRP) was raised in goat using a synthetic peptide representing amino acid residues 410-419 (EQKLISEEDL) of human myc conjugated to KLH as the immunogen.
MYC
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
7-00E-10
unconjugated
Application Notes
ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
Supplied as a liquid in 50 mM Hepes buffer, pH 7.1, with 0.0 NaCl, with 0.2 % BSA, and 0.01 % thimerasol.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
Myc gene encodes a transcription factor that is believed to regulate expression of 15% of all genes through binding of Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). This means that in addition to its role as a classical transcription factor, Myc also functions to regulate global chromatin structure.