IFNB1 antibody (Biotin)

Catalog No. ABIN465657

Product Details anti-IFNB1 Antibody (Biotin)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human IFN-beta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IFN- beta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IFN-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-beta. To detect Human IFN-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IFN-beta (LS-C104330) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-beta. Western Blot: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IFNB1

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: IFN Beta / Interferon Beta (IFNB1 Products)

Synonyms: IFB antibody, IFF antibody, IFNB antibody, IFN-beta antibody, Ifb antibody, Ifnb antibody, IFNb antibody, interferon beta 1 antibody, interferon beta 1, fibroblast antibody, interferon, beta 1, fibroblast antibody, IFNB1 antibody, Ifnb1 antibody

Background: Name/Gene ID: IFNB1
Family: Interferon

Synonyms: IFNB1, IFF, IFB, IFNB, Interferon beta, Interferon, beta 1, fibroblast, Fibroblast interferon, IFN-beta

Gene ID: 3456

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome