anti-Dog (Canine) Integrin beta 2 antibody for Immunoprecipitation

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • ITGB2
  • 2E6
  • AI528527
  • Cd18
  • LAD
  • LCAMB
  • Lfa1
  • MF17
  • CD18
  • LFA-1
  • MAC-1
  • MFI7
  • AI256028
  • LTbetaR
  • Ltar
  • TNF-R-III
  • TNFCR
  • TNFR-RP
  • TNFR2-RP
  • TNFRrp
  • Tnfbr
  • Tnfrsf3
  • D12S370
  • LT-BETA-R
  • TNFR3
  • TNFRSF3
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • integrin, beta 2
  • integrin beta 2
  • lymphotoxin B receptor
  • lymphotoxin beta receptor (TNFR superfamily, member 3)
  • ITGB2
  • Itgb2
  • CD18
  • Ltbr
  • LTBR
Reactivity
Dog (Canine), Human
490
216
118
43
26
21
21
5
4
3
3
3
3
3
2
1
1
1
Host
Rat
362
164
162
15
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
88
74
53
20
15
12
9
8
8
7
7
7
6
6
6
6
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
487
291
202
147
94
90
75
68
53
40
28
18
11
9
7
7
6
2
1
1
1
1
1
1
1
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Catalog No. ABIN118553
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2478872 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 4
1 ABIN118554 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
1 ABIN319696 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 3
1 ABIN2478878 Func IHC (fro) FACS IP Rat IgG2b Log in to see YFC118-3 4
1 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
1 ABIN4327005 FACS ICC IF IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3
1 ABIN576530 BR FACS IHC (fro) IP PE Rat IgG2b Log in to see
1 ABIN600732 IHC (fro) FACS IP Rat IgG2b Log in to see
1 ABIN206735 BR FACS IHC (fro) IP Biotin Rat IgG2b Log in to see
1 ABIN217662 BR FACS IHC (fro) IP FITC Rat IgG2b Log in to see
1 ABIN206736 BR FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3
1 ABIN1820130 BR FACS Func IHC (fro) IP Rat IgG2b Log in to see YFC118-3
1 ABIN4327020 FACS IHC IHC (fro) IP PerCP Rat IgG2b Log in to see YFC118-3
1 ABIN477983 FACS IP Rat IgG2c Log in to see YKIX490-6-4
1 ABIN305537 FACS IP FITC Rat IgG2c Log in to see
1 ABIN1834920 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(490), (216), (118), (43), (26), (21), (21), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rat
(362), (164), (162), (15), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(88), (74), (53), (20), (15), (12), (9), (8), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(487), (291), (202), (147), (94), (90), (75), (68), (53), (40), (28), (18), (11), (9), (7), (7), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118553) Images
Specificity This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Purification Purified
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118553) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 is expressed by the majority of leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN118553) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/1000 - 1/5000. Recommended positive control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN118553) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 antibody (ABIN118553)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118553) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 (ITGB2) antibody (ABIN118553) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD18