anti-Dog (Canine) Integrin beta 2 antibody for Immunoprecipitation

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Reactivity
Dog (Canine), Human
515
206
120
38
37
23
22
13
4
4
3
3
3
3
3
2
1
1
Host
Rat
390
174
151
9
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
83
73
50
28
14
12
11
10
8
8
7
7
6
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
491
294
185
178
99
91
80
76
65
40
28
15
13
11
7
6
4
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118553
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2478872 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 4
1 ABIN319696 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 3
1 ABIN118554 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
1 ABIN2478878 Func IHC (fro) FACS IP Rat IgG2b Log in to see YFC118-3 4
1 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
1 ABIN600732 FACS IHC IHC (fro) IP Rat IgG2b Log in to see 0
1 ABIN4327005 FACS ICC IF IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3 0
1 ABIN576530 BR FACS IHC IHC (fro) IP PE Rat IgG2b Log in to see 0
1 ABIN206736 BR FACS IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3 0
1 ABIN1820130 BR FACS Func IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3 0
1 ABIN4327020 FACS IHC IHC (fro) IP PerCP Rat IgG2b Log in to see YFC118-3 0
1 ABIN217662 BR FACS IHC IHC (fro) IP FITC Rat IgG2b Log in to see 0
1 ABIN1834920 FACS IF IHC IHC (fro) IP Mouse IgG1 Log in to see CA1-4E9 0

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(515), (206), (120), (38), (37), (23), (22), (13), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rat
(390), (174), (151), (9), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(83), (73), (50), (28), (14), (12), (11), (10), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(491), (294), (185), (178), (99), (91), (80), (76), (65), (40), (28), (15), (13), (11), (7), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118553) Images
Specificity This antibody recognises the CD18 cell surface antigen, the integrin b2 subunit. This antibody blocks CD18 mediated homotypic adhesion.
Purification Purified
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the rat Y3/Ag.1.2.3 myeloma cell line.
Clone YFC118-3
Isotype IgG2b
Plasmids, Primers & others

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118553) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background CD18 is expressed by the majority of leucocytes.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN118553) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/1000 - 1/5000. Recommended positive control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN118553) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 antibody (ABIN118553)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118553) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 (ITGB2) antibody (ABIN118553) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD18