anti-Mouse (Murine) Integrin beta 2 antibody for Blocking Reagent

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Reactivity
Mouse (Murine)
471
206
117
38
38
21
20
13
5
4
3
3
3
3
3
2
1
1
Host
Rat
359
161
152
10
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is un-conjugated
82
72
50
27
14
11
10
8
8
8
7
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
465
281
186
141
94
91
77
74
55
40
27
16
10
9
7
6
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN2689033
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689032 BR IHC (fro) FACS IP Rat IgG1, kappa Log in to see GAME 3
1 ABIN2663105 BR FACS PE Rat IgG2a kappa Log in to see M18-2 9
1 ABIN2663106 BR FACS PE Rat IgG2a kappa Log in to see M18-2 9
1 ABIN1176975 BR Func IHC (f) IHC (fro) FACS Rat IgG2a, kappa Log in to see M18-2 6
1 ABIN490178 BR FACS Func IHC (fro) IHC (p) IP Neut WB Rat IgG2a Log in to see M18-2 0
1 ABIN490150 BR FACS Func IHC (fro) IP Neut WB FITC Rat IgG2a Log in to see M18-2 0
1 ABIN490166 BR FACS Func IHC (fro) IP Neut WB Rat IgG2a Log in to see M18-2 0
1 ABIN1176955 BR IHC (fro) FACS IP Rat IgG1, kappa Log in to see GAME-46 3

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Mouse (Murine)
(471), (206), (117), (38), (38), (21), (20), (13), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rat
(359), (161), (152), (10), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is un-conjugated
(82), (72), (50), (27), (14), (11), (10), (8), (8), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(465), (281), (186), (141), (94), (91), (77), (74), (55), (40), (27), (16), (10), (9), (7), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN2689033) Images
Brand BD Pharmingen™
Characteristics The M18/2 antibody reacts with the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αXβ2 integrin). Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The β2 integrins are important mediators of leukocyte-endothelium interactions. It has been reported that M18/2 antibody blocks in vivo metastasis of the LB lymphoma to the spleen and that it blocks in vitro formation of aggregates of LB cells and splenocytes. However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts. Recent in vitro studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3.

BD Pharmingen™ Purified Rat Anti-Mouse CD18 - Purified - Clone M18/2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse CTL glycoproteins
Clone M18-2
Isotype IgG2a, kappa

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN2689033) Images back to top
Antigen
Alternative Name CD18 (ITGB2 Antibody Abstract)
Background Synonyms: Integrin β2 chain
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN2689033) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN2689033) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Integrin beta 2 antibody (ABIN2689033)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Product cited in:

Driessens, van Hulten, Zuurbier, La Rivière, Roos: "Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct." in: Journal of leukocyte biology, Vol. 60, Issue 6, pp. 758-65, 1997

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994

Zahalka, Naor: "Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness." in: International immunology, Vol. 6, Issue 6, pp. 917-24, 1994

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993

Isobe, Yagita, Okumura, Ihara: "Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1." in: Science (New York, N.Y.), Vol. 255, Issue 5048, pp. 1125-7, 1992

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983