anti-Human PDK4 antibody for Enzyme Immunoassay

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
  • pyruvate dehydrogenase kinase 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pdk4
  • PDK4
  • Pdk4
Epitope
Middle Region
29
20
18
12
12
12
10
10
8
6
6
6
6
5
5
4
2
2
1
1
1
1
1
1
Reactivity
Human
137
70
14
2
2
2
1
1
1
1
1
Host
Rabbit
139
20
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
8
7
7
7
6
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
145
81
61
37
25
17
4
3
1
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Catalog No. ABIN359099
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359100 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Log in to see Polyclonal 4
1 ABIN5610925 EIA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 0
1 ABIN4620385 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope Middle Region
(29), (20), (18), (12), (12), (12), (10), (10), (8), (6), (6), (6), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(137), (70), (14), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(139), (20)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(11), (8), (7), (7), (7), (6), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(145), (81), (61), (37), (25), (17), (4), (3), (1)
Pubmed 4 references available
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling References for anti-PDK4 antibody (ABIN359099) Images
Specificity This antibody reacts to PDK4.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human PDK4.
Isotype Ig Fraction

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling References for anti-PDK4 antibody (ABIN359099) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase lipoamide kinase isozyme 4 mitochondrial
Gene ID 5166, 9606
UniProt Q16654
Pathways PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling References for anti-PDK4 antibody (ABIN359099) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details References for anti-PDK4 antibody (ABIN359099) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDK4 antibody (ABIN359099)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Background publications

Rosa, Di Rocco, Manco, Greco, Castagneto, Vidal, Mingrone: "Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients." in: Obesity research, Vol. 11, Issue 2, pp. 176-82, 2003

Razeghi, Young, Ying, Depre, Uray, Kolesar, Shipley, Moravec, Davies, Frazier, Taegtmeyer: "Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading." in: Cardiology, Vol. 97, Issue 4, pp. 203-9, 2002

Rowles, Scherer, Xi, Majer, Nickle, Rommens, Popov, Harris, Riebow, Xia, Tsui, Bogardus, Prochazka: "Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human." in: The Journal of biological chemistry, Vol. 271, Issue 37, pp. 22376-82, 1996

Gudi, Bowker-Kinley, Kedishvili, Zhao, Popov: "Diversity of the pyruvate dehydrogenase kinase gene family in humans." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28989-94, 1996

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling References for anti-PDK4 antibody (ABIN359099) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (Middle Region) antibody (ABIN359099) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4, ...
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (Middle Region) antibody (ABIN359099) Western blot analysis of PDK4 Antibody in CEM cell line lysates (35ug/lane). PDK4 (ar...