anti-Human PDK4 antibody for Western Blotting

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
  • pyruvate dehydrogenase kinase 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pdk4
  • PDK4
  • Pdk4
Epitope
AA 382-410, C-Term
24
19
16
12
12
10
10
9
8
7
7
6
5
5
5
4
4
3
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
146
76
19
2
2
2
1
1
1
1
1
Host
Rabbit
149
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
12
9
7
7
7
7
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
154
76
71
35
25
17
7
4
3
1
Options
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Catalog No. ABIN391039
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1721978 ABIN446469 ICC IF WB Rabbit AA 100-150, Internal Region Log in to see Polyclonal 8
3.1721978 ABIN2777592 IHC WB Rabbit N-Term Log in to see Polyclonal 2
3.1721978 ABIN3043461 WB Rabbit IgG AA 91-125, N-Term Log in to see Polyclonal 0
3.1721978 ABIN359100 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Log in to see Polyclonal 4
3.1721978 ABIN359099 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Log in to see Polyclonal 4
3.1721978 ABIN446476 ICC IF WB Rabbit AA 200-250, Internal Region Log in to see Polyclonal 1
3.1721978 ABIN5537300 IF IHC (p) WB Rabbit Ig Fraction AA 83-111 Log in to see Polyclonal 0
3.1721978 ABIN5537298 IF IHC (p) WB Rabbit Ig Fraction AA 382-410, C-Term Log in to see Polyclonal 0
3.1721978 ABIN1874085 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1721978 ABIN1539836 WB Mouse IgG1 Log in to see 345CT19-6-2 0
3.1721978 ABIN4620385 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
3.1721978 ABIN1860174 ICC IHC WB Rabbit IgG AA 73-316 Log in to see Polyclonal 0
3.1721978 ABIN391041 IF IHC (p) WB Rabbit Ig Fraction AA 83-111, Asp98, Center Log in to see Polyclonal 0
3.1721978 ABIN391040 IHC (p) WB Rabbit Ig Fraction AA 250-277, Glu265 Log in to see Polyclonal 0
3.1721978 ABIN2460574 IHC ELISA WB Rabbit Log in to see Polyclonal 0
3.1721978 ABIN4344604 ICC IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
3.1721978 ABIN4890316 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal 0
3.1721978 ABIN6266305 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1721978 ABIN631050 IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.1721978 ABIN5585455 WB Rabbit Log in to see Polyclonal 0

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope AA 382-410, C-Term
(24), (19), (16), (12), (12), (10), (10), (9), (8), (7), (7), (6), (5), (5), (5), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(146), (76), (19), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(149), (19)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PDK4 antibody is un-conjugated
(12), (9), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(154), (76), (71), (35), (25), (17), (7), (4), (3), (1)
Pubmed 24 references available
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling References for anti-PDK4 antibody (ABIN391039) Images
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 AA from the C-terminal region of human PDK4.
Clone RB03041
Isotype Ig Fraction
Plasmids, Primers & others

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling References for anti-PDK4 antibody (ABIN391039) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background Function
Serine/threonine kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Cellular Location
Mitochondrion matrix.

Tissue Location
Ubiquitous, highest levels of expression in heart and skeletal muscle

Synonyms: Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase kinase isozyme 4, mitochondrial
Molecular Weight 46469 Da
Gene ID 5166
UniProt Q16654
Pathways PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling References for anti-PDK4 antibody (ABIN391039) Images back to top
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:1000, IHC (p) = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details References for anti-PDK4 antibody (ABIN391039) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-PDK4 antibody (ABIN391039)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Product cited in:

Cole, Abd Jamil, Heather, Murray, Sutton, Slingo, Sebag-Montefiore, Tan, Aksentijević, Gildea, Stuckey, Yeoh, Carr, Evans, Aasum, Schofield, Ratcliffe, Neubauer, Robbins, Clarke: "On the pivotal role of PPARα in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 8, pp. 2684-97, 2017 Method employed by authors: Western Blotting (WB)

Zhang, Wu, Choiniere, Yang, Huang, Bennett, Wang: "Arsenic silences hepatic PDK4 expression through activation of histone H3K9 methylatransferase G9a." in: Toxicology and applied pharmacology, Vol. 304, pp. 42-7, 2017 Method employed by authors: Western Blotting (WB)

Zhang, Zhang, Geng, Yi, Huo, Talmon, Kim, Wang, Wang: "Transforming Growth Factor β Mediates Drug Resistance by Regulating the Expression of Pyruvate Dehydrogenase Kinase 4 in Colorectal Cancer." in: The Journal of biological chemistry, Vol. 291, Issue 33, pp. 17405-16, 2017 Method employed by authors: Western Blotting (WB)

Li, Li, Hu, Lei, Xiong, Ji, Yin, Wei, Hu: "The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis." in: Oncogene, Vol. 36, Issue 7, pp. 989-998, 2017 Method employed by authors: Western Blotting (WB)

Jiang, de Castro Barbosa, Massart, Deshmukh, Löfgren, Duque-Guimaraes, Ozilgen, Osler, Chibalin, Zierath: "Diacylglycerol kinase-δ regulates AMPK signaling, lipid metabolism, and skeletal muscle energetics." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 1, pp. E51-60, 2016 Method employed by authors: Western Blotting (WB)

Mazar, Qi, Lee, Marchica, Govindarajan, Shelley, Li, Ray, Perera: "MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells." in: Molecular and cellular biology, Vol. 36, Issue 7, pp. 1090-108, 2016 Method employed by authors: Western Blotting (WB)

Walter, Thomalla, Bruhn, Fagan, Zehowski, Yee, Skildum: "Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cells." in: SpringerPlus, Vol. 4, pp. 689, 2015 Method employed by authors: Western Blotting (WB)

Kamarajugadda, Stemboroski, Cai, Simpson, Nayak, Tan, Lu: "Glucose oxidation modulates anoikis and tumor metastasis." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1893-907, 2012 Method employed by authors: Western Blotting (WB)

Haldar, Jeyaraj, Anand, Zhu, Lu, Prosdocimo, Eapen, Kawanami, Okutsu, Brotto, Fujioka, Kerner, Rosca, McGuinness, Snow, Russell, Gerber, Bai, Yan, Nosek, Brotto, Hoppel, Jain: "Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 17, pp. 6739-44, 2012 Method employed by authors: Western Blotting (WB)

Williams, Wan, Frier, Bell, Field, Wright: "Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 302, Issue 1, pp. R49-58, 2011 Method employed by authors: Western Blotting (WB)

Long, Kostovski, Boon, Hjeltnes, Krook, Widegren: "Differential expression of metabolic genes essential for glucose and lipid metabolism in skeletal muscle from spinal cord injured subjects." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 110, Issue 5, pp. 1204-10, 2011 Method employed by authors: Western Blotting (WB)

Phillips, Aponte, Covian, Balaban: "Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes." in: Biochemistry, Vol. 50, Issue 13, pp. 2515-29, 2011 Method employed by authors: Western Blotting (WB)

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, 2011 Method employed by authors: Western Blotting (WB)

Attia, Connnaughton, Boone, Wang, Elam, Ness, Cook, Park: "Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha)." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2375-85, 2010 Method employed by authors: Western Blotting (WB)

Dateki, Kunitomo, Yoshioka, Yanai, Nakasono, Negishi: "Adaptive gene regulation of pyruvate dehydrogenase kinase isoenzyme 4 in hepatotoxic chemical-induced liver injury and its stimulatory potential for DNA repair and cell proliferation." in: Journal of receptor and signal transduction research, Vol. 31, Issue 1, pp. 85-95, 2010

Zhao, Okuyama, Hashimoto, Tagawa, Jomori, Yang: "Bezafibrate induces myotoxicity in human rhabdomyosarcoma cells via peroxisome proliferator-activated receptor alpha signaling." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 24, Issue 1, pp. 154-9, 2010 Method employed by authors: Western Blotting (WB)

Basu, Oudit, Wang, Zhang, Ussher, Lopaschuk, Kassiri: "Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 6, pp. H2096-108, 2009 Method employed by authors: Western Blotting (WB)

Hsieh, Das, Sambandam, Zhang, Nahlé: "Regulation of the PDK4 isozyme by the Rb-E2F1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 41, pp. 27410-7, 2008 Method employed by authors: Western Blotting (WB)

Nahlé, Hsieh, Pietka, Coburn, Grimaldi, Zhang, Das, Abumrad: "CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14317-26, 2008 Method employed by authors: Western Blotting (WB)

Long, Glund, Garcia-Roves, Zierath: "Calcineurin regulates skeletal muscle metabolism via coordinated changes in gene expression." in: The Journal of biological chemistry, Vol. 282, Issue 3, pp. 1607-14, 2007 Method employed by authors: Western Blotting (WB)

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling References for anti-PDK4 antibody (ABIN391039) back to top
Supplier Images
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410), (C-Term) antibody (ABIN391039) The PDK4 Antibody (C-term) (ABIN391039) is used in Western blot to detect PDK4 in mou...
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410), (C-Term) antibody (ABIN391039) Western blot analysis of PDK4 (arrow) using PDK4 Antibody (C-term) (ABIN391039). 293 ...
Immunohistochemistry (IHC) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410), (C-Term) antibody (ABIN391039) Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410), (C-Term) antibody (ABIN391039) ABIN391039,ABIN2841206 staining PDK4 in human heart tissue sections by Immunohistoche...
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410), (C-Term) antibody (ABIN391039) All lanes : Anti-PDK4 Antibody (C396) at 1:2000 dilution Lane 1: human heart lysates ...
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410), (C-Term) antibody (ABIN391039) Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate Lysates/proteins ...