Collagen Type I antibody

Catalog No. ABIN126040

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody detects exhibits the following reactivities in ELISA both native and denaturated Collagen types: Collagen types 1 and 3: 100 % . Collagen types 4 and 5: 90 %. Collagen type 2: 80 %. Fibronectin, Albumin and Immunoglobulins all < 0.5 %.

Cross-Reactivity (Details): Also reacts to: type II, and V, III, IV

Purification: Affinity Chromatography on immobilized collagen types I-V

Immunogen: A mixture of human collagen 1-5 from human placenta.

Application Details

Application Notes: Immunohistochemistry on Frozen Sections (1/10-1/40). ELISA (1/2000-1/8000). Immunoflourescence (1/10-1/40).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.5 containing 0.1 % Mannitol and 0.1 % Dextran and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte. Collagen III occurs in most soft connective tissues along with collagen I. Collagen IV is the major structural component of glomerular basement membranes, forming a Chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452