Collagen Type I antibody
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- Target See all Collagen Type I (COL1) Antibodies
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Collagen Type I antibody is un-conjugated
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)
- Specificity
- Human Collagen type I: 100 % Collagen type II and IV: < 0.1 % Collagen type III and V: < 1.0 % Human Fibronectin: < 0.1 %.
- Cross-Reactivity (Details)
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Species reactivity (expected):Cattle (< 1.0 %), Rat and Chicken (< 0.1 %).
Species reactivity (tested):Human. - Purification
- Affinity Chromatography
- Immunogen
- Purified Collagen type I from Human skin.
- Top Product
- Discover our top product COL1 Primary Antibody
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- Application Notes
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RIA. ELISA: >/= 1/200 (OD >/= 500). Immunflourescence Assays: 1/80. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Human skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water.
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered solution, no BSA and preservative added.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- Alternative Name
- Collagen Type I (COL1 Products)
- Synonyms
- collagen type III alpha 1 chain antibody, COL3A1 antibody
- Background
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
- Gene ID
- 1277
- NCBI Accession
- NP_000079
- UniProt
- P02452
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