Collagen Type I antibody
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- Target See all Collagen Type I (COL1) Antibodies
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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Reactivity
- Human, Mammalian
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Collagen Type I antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Specificity
- This antibody recognizes Collagen type I.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mammal.
Species reactivity (tested):Human. - Purification
- Immunoaffinity Chromatography.
- Immunogen
- Collagen Type I from Human and Bovine Placenta
- Top Product
- Discover our top product COL1 Primary Antibody
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- Application Notes
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ELISA: 1/5,000-1/50,000. Immunoprecipitation. Western blot: 1/5,000-1/50,000. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 containing 0.01 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- Alternative Name
- Collagen Type I (COL1 Products)
- Synonyms
- collagen type III alpha 1 chain antibody, COL3A1 antibody
- Background
- Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I Collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
- Gene ID
- 1277
- NCBI Accession
- NP_000079
- UniProt
- P02452
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