anti-Mouse (Murine) AIFM1 antibody for Western Blotting

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • AIF
  • CMTX4
  • COWCK
  • COXPD6
  • PDCD8
  • CG7263
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • aif
  • pdcd8
  • AIFM1
  • PCD8
  • AIFsh2
  • Hq
  • Pdcd8
  • mAIF
  • Aif
  • zgc:91994
  • apoptosis inducing factor mitochondria associated 1
  • allograft inflammatory factor 1
  • Apoptosis inducing factor
  • apoptosis-inducing factor 1, mitochondrial
  • apoptosis inducing factor
  • apoptosis inducing factor, mitochondria associated 1
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • apoptosis-inducing factor, mitochondrion-associated 1
  • AIFM1
  • AIF1
  • AIF
  • LOC412212
  • aif
  • aifm1
  • Aifm1
Epitope
C-Term
29
19
19
15
12
11
8
7
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
241
108
101
29
20
18
16
11
9
8
7
7
3
2
2
1
1
Host
Rabbit
195
37
14
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
6
6
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
224
99
81
54
46
34
24
18
13
13
8
6
3
2
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Catalog No. ABIN2778665
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357207 ABIN724010 IF (p) IHC (p) WB Rabbit IgG AA 130-180 Log in to see Polyclonal 2
3.1357207 ABIN222988 WB Rabbit IgG Log in to see Polyclonal 2
3.1357207 ABIN3043779 ICC IHC (p) WB Rabbit IgG AA 582-613, C-Term Log in to see Polyclonal 0
3.1357207 ABIN2792122 WB Rabbit Middle Region Log in to see Polyclonal 1
3.1357207 ABIN968950 ICC FACS IHC ELISA WB Mouse IgG2b Log in to see 4-00E-07 1
3.1357207 ABIN4880194 ELISA FACS ICC IHC WB Mouse IgG2b Log in to see 4E7 0
3.1357207 ABIN2707315 IC IF IP IHC WB Rabbit N-Term Log in to see Polyclonal 0
3.1357207 ABIN1845959 ELISA FACS IF/ICC IHC WB Mouse IgG2b Log in to see 0
3.1357207 ABIN1534424 ELISA WB Rabbit IgG AA 51-100 Log in to see Polyclonal 2
3.1357207 ABIN4278820 ELISA FACS ICC IF IHC WB Mouse IgG2b Log in to see 4E7 0
3.1357207 ABIN6259842 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN4278849 ELISA WB Goat C-Term, Isoform 1 Log in to see Polyclonal 1
3.1357207 ABIN1870886 IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN3180477 ELISA IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.1357207 ABIN5949302 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 500-600 Log in to see SZ05-01 0
3.1357207 ABIN2974032 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN1105269 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-007 1
3.1357207 ABIN5965207 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357207 ABIN3044471 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 596-613, C-Term Log in to see Polyclonal 0
3.1357207 ABIN261023 IHC (p) IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope C-Term
(29), (19), (19), (15), (12), (11), (8), (7), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(241), (108), (101), (29), (20), (18), (16), (11), (9), (8), (7), (7), (3), (2), (2), (1), (1)
Host Rabbit
(195), (37), (14), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(6), (6), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(224), (99), (81), (54), (46), (34), (24), (18), (13), (13), (8), (6), (3), (2)
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN981498
Sequence VDSSLPTVGV FAKATAQDNP KSATEQSGTG IRSESETESE ASEITIPPST
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against PDCD8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human PDCD8
Plasmids, Primers & others

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDCD8 (AIFM1 Antibody Abstract)
Background AIFM1 (PDCD8) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, AIFM1 induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.
Alias Symbols: AIF, PDCD8, COXPD6
Protein Interaction Partner: ISG15, STAU1, BAG6, UBC, NEDD8, MDM2, ASB12, RNF2, BMI1, SUZ12, PARK2, BAG3, ADRB2, HDAC11, OXSR1, ILK, PAN2, NOS2, MLH1, CFTR, ESR1, TST, FSCN1, CPOX, SHC1, FBXO6, TSC22D4, CAND1, COPS5, CUL1, CUL2, CUL3, CUL5, TONSL, BLNK, PIDD1, XIAP, AMFR, Tubb4b, Tub
Protein Size: 326
Molecular Weight 36 kDa
Gene ID 9131
NCBI Accession NM_145813, NP_665812
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 326 AA

Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2778665) WB Suggested Anti-PDCD8 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2778665) WB Suggested Anti-PDCD8 Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.2500 Positi...
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2778665) Host: Rabbit Target Name: AIFM1 Sample Tissue: Mouse Liver Antibody Dilution: ...
Immunohistochemistry (IHC) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2778665) Rabbit Anti-AIFM1 Antibody Formalin Fixed Paraffin Embedded Tissue: Human heart Tis...
Immunohistochemistry (IHC) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2778665) Rabbit Anti-AIFM1 Antibody Formalin Fixed Paraffin Embedded Tissue: Human Kidney Ti...