anti-Pig (Porcine) AIFM1 antibody for Western Blotting

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • AIF
  • CMTX4
  • COWCK
  • COXPD6
  • PDCD8
  • CG7263
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • aif
  • pdcd8
  • AIFM1
  • PCD8
  • AIFsh2
  • Hq
  • Pdcd8
  • mAIF
  • Aif
  • zgc:91994
  • apoptosis inducing factor mitochondria associated 1
  • allograft inflammatory factor 1
  • Apoptosis inducing factor
  • apoptosis-inducing factor 1, mitochondrial
  • apoptosis inducing factor
  • apoptosis inducing factor, mitochondria associated 1
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • apoptosis-inducing factor, mitochondrion-associated 1
  • AIFM1
  • AIF1
  • AIF
  • LOC412212
  • aif
  • aifm1
  • Aifm1
Epitope
C-Term
29
20
16
15
12
11
8
7
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
243
107
101
29
21
19
16
12
10
8
8
7
4
2
2
1
1
1
Host
Goat
199
36
13
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
6
6
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
226
100
86
54
46
34
24
18
13
13
8
6
3
2
1
Options
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Available images

Catalog No. ABIN2630015
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.235657 ABIN724010 IF (p) IHC (p) WB Rabbit IgG AA 130-180 Log in to see Polyclonal 2
19.735657 ABIN5893605 ELISA IF/ICC WB Goat Log in to see Polyclonal 0
16.735657 ABIN2778665 IHC WB Rabbit C-Term Log in to see Polyclonal 0
13.735656 ABIN2560986 ELISA WB Goat IgG Log in to see Polyclonal 0
10.735656 ABIN2792122 WB Rabbit Middle Region Log in to see Polyclonal 1
10 ABIN462451 ELISA ICC IHC IHC (p) WB Rabbit IgG AA 593-606 Log in to see Polyclonal 0
7.1922464 ABIN4278849 ELISA WB Goat C-Term, Isoform 1 Log in to see Polyclonal 1
7 ABIN214083 IHC IHC (p) WB Rabbit IgG AA 211-260 Log in to see Polyclonal 0
7 ABIN305789 IHC IHC (p) WB Rabbit AA 593-606 Log in to see Polyclonal 0
7 ABIN957132 IHC IHC (fro) IHC (p) IP WB Rabbit AA 151-169 Log in to see Polyclonal 0
4 ABIN470368 WB Rabbit C-Term Log in to see Polyclonal 0
4 ABIN2470307 IHC WB Rabbit AA 593-606 Log in to see Polyclonal 0
1 ABIN211058 WB Rabbit IgG AA 593-606 Log in to see Polyclonal 0
1 ABIN724019 IHC (p) WB HRP Rabbit IgG AA 130-180 Log in to see Polyclonal 0
1 ABIN2615248 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 596-613 Log in to see Polyclonal 0
1 ABIN2738204 IHC IHC (p) WB Rabbit IgG AA 582-613 Log in to see Polyclonal 0
1 ABIN724012 IHC (p) WB Biotin Rabbit IgG AA 130-180 Log in to see Polyclonal 0

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope C-Term
(29), (20), (16), (15), (12), (11), (8), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(243), (107), (101), (29), (21), (19), (16), (12), (10), (8), (8), (7), (4), (2), (2), (1), (1), (1)
Host Goat
(199), (36), (13), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(6), (6), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Western Blotting (WB)
(226), (100), (86), (54), (46), (34), (24), (18), (13), (13), (8), (6), (3), (2), (1)
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling Images
Purpose AIFM1
Blocking Peptide Blocking peptide for this product available: ABIN2815154
Sequence NEVAKLFNIH ED
Specificity This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
Predicted Reactivity Human, Mouse, Rat, Dog, Pig, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence
Plasmids, Primers & others

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AIFM1 (AIFM1 Antibody Abstract)
Background AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
Gene ID 9131
NCBI Accession NP_004199, NP_665811, NP_665812, NP_001124318
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling Images back to top
Application Notes

DS WB Results: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.01µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Dete...
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.01µg/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35µg...
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015 (0.03µg/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35µg protein...
Immunofluorescence (IF) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015-P1 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, perm...
Immunofluorescence (IF) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN2630015) ABIN2630015-P1 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, perm...