anti-Rat (Rattus) AIFM1 antibody for Immunohistochemistry (Frozen Sections)

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • AIF
  • CMTX4
  • COWCK
  • COXPD6
  • PDCD8
  • CG7263
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • aif
  • pdcd8
  • AIFM1
  • PCD8
  • AIFsh2
  • Hq
  • Pdcd8
  • mAIF
  • Aif
  • zgc:91994
  • apoptosis inducing factor mitochondria associated 1
  • allograft inflammatory factor 1
  • Apoptosis inducing factor
  • apoptosis-inducing factor 1, mitochondrial
  • apoptosis inducing factor
  • apoptosis inducing factor, mitochondria associated 1
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • apoptosis-inducing factor, mitochondrion-associated 1
  • AIFM1
  • AIF1
  • AIF
  • LOC412212
  • aif
  • aifm1
  • Aifm1
Epitope
AA 596-613, C-Term
29
19
17
15
12
11
8
7
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
240
107
101
30
21
19
16
12
10
8
8
7
4
2
2
1
1
1
Host
Rabbit
194
37
14
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
6
6
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
223
98
82
53
48
33
24
18
13
13
7
6
3
2
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Catalog No. ABIN3044471
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN3029686 ICC IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN957132 IHC IHC (fro) IHC (p) IP WB Rabbit AA 151-169 Log in to see Polyclonal 0
1 ABIN2615248 ICC IHC IHC (fro) IHC (p) WB Rabbit IgG AA 596-613 Log in to see Polyclonal 0
-2.020279 ABIN4278826 IHC (fro) IHC (p) IP SimWes WB Rabbit AA 151-170 Log in to see Polyclonal 0
-11.020279 ABIN4278825 IHC (fro) IHC (p) IP SimWes WB Rabbit AA 151-170 Log in to see Polyclonal 0

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope AA 596-613, C-Term
(29), (19), (17), (15), (12), (11), (8), (7), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(240), (107), (101), (30), (21), (19), (16), (12), (10), (8), (8), (7), (4), (2), (2), (1), (1), (1)
Host Rabbit
(194), (37), (14), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(6), (6), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(223), (98), (82), (53), (48), (33), (24), (18), (13), (13), (7), (6), (3), (2)
Pubmed 3 references available
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling References for anti-AIFM1 antibody (ABIN3044471) Images
Purpose Rabbit IgG polyclonal antibody for Apoptosis-inducing factor 1, mitochondrial(AIFM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Sequence EQHEDLNEVA KLFNIHED
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Apoptosis-inducing factor 1, mitochondrial(AIFM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: apoptosis-inducing factor, mitochondrion-associated, 1
Protein Name: Apoptosis-inducing factor 1, mitochondrial
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human AIF(596-613aa EQHEDLNEVAKLFNIHED), identical to the related rat and mouse sequences.
Isotype IgG
Plasmids, Primers & others

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling References for anti-AIFM1 antibody (ABIN3044471) Images back to top
Antigen
Alternative Name AIFM1 (AIFM1 Antibody Abstract)
Background Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.

Synonyms: AIFM1 antibody|Apoptosis inducing factor 1, mitochondrial antibody|Apoptosis inducing factor antibody|Apoptosis inducing factor, mitochondrion associated, 1 antibody|Apoptosis-inducing factor 1 antibody|CMTX4 antibody|COXPD6 antibody|Harlequin antibody|Hq antibody|mAIF antibody|MGC111425 antibody|MGC5706 antibody|mitochondrial antibody|PDCD 8 antibody|PDCD8 antibody|Programmed cell death 8(apoptosis inducing factor) antibody|Programmed cell death 8 antibody|Programmed cell death 8 isoform 1 antibody|Programmed cell death 8 isoform 2 antibody|Programmed cell death 8 isoform 3 antibody|Programmed cell death 8 isoform 5 antibody|Programmed cell death protein 8 antibody|Programmed cell death protein 8 mitochondrial antibody|Programmed cell death protein 8 mitochondrial precursor antibody|Striatal apoptosis inducing factor antibody
UniProt O95831
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling References for anti-AIFM1 antibody (ABIN3044471) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details References for anti-AIFM1 antibody (ABIN3044471) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-AIFM1 antibody (ABIN3044471)

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling Images back to top
Product cited in:

Jin, Duan, Xu, Ge, Li, Qiu, Song, Cao, Wang: "Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture." in: Biomedical reports, Vol. 5, Issue 1, pp. 73-78, 2016

Yang, Zhang, Wu, Shi, Xue, Li, Chen, Zhu, Wei, He, Liu: "Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway." in: Stem cells international, Vol. 2016, pp. 1690896, 2016

Feng, Liu, Li, Li: "Protective effects of nigranoic acid on cerebral ischemia-reperfusion injury and its mechanism involving apoptotic signaling pathway." in: Cell biochemistry and biophysics, Vol. 71, Issue 1, pp. 345-51, 2015

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling References for anti-AIFM1 antibody (ABIN3044471) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 596-613), (C-Term) antibody (ABIN3044471) Anti-AIF antibody, IHC(P) IHC(P): Rat Cardiac Muscle Tissue
Western Blotting (WB) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 596-613), (C-Term) antibody (ABIN3044471) Anti-AIF antibody, Western blotting Lane 1: Rat Heart Tissue Lysate Lane 2: Rat Brain...
Immunohistochemistry (IHC) image for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 596-613), (C-Term) antibody (ABIN3044471) Anti-AIF antibody, IHC(P) IHC(P): Human Lung Cancer Tissue