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SARS-CoV-2 Spike Protein (B.1.617.2 - delta, Trimer) (His tag)

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Catalog No. ABIN7012937
  • Target See all SARS-CoV-2 Spike Proteins
    SARS-CoV-2 Spike
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    B.1.617.2 - delta, Trimer
    Origin
    • 180
    • 37
    • 8
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta
    Source
    • 164
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike protein is labelled with His tag.
    Characteristics
    SARS-CoV-2 Spike Trimer, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617.2). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    Purity
    >95 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.This product is stable after storage at:
    4-8°C for 12 months in lyophilized state,
    -70°C for 3 months under sterile conditions after reconstitution.
  • Target
    SARS-CoV-2 Spike
    Abstract
    SARS-CoV-2 Spike Products
    Synonyms
    E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
    Target Type
    Viral Protein
    Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    137.8 kDa
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