SARS-CoV-2 Spike Protein (B.1.640, RBD) (His tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Protein Characteristics
- B.1.640, RBD
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with His tag.
- Purpose
- SARS-Cov-2 Spike RBD (B.1.640.2/IHU) Protein
- Sequence
- Arg319-Phe541 (R346S, N394S, Y449N, E484K, F490S, N501Y)
- Characteristics
- Recombinant SARS-Cov-2 Spike RBD(B.1.640.2/IHU) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Background
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 25.76 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
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