SARS-CoV-2 Spike Protein (His-Avi Tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.
- Characteristics
- DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188] R683A (accession # QHD43416.1) in 3 replicates including a C-terminal His AVI tag was expressed in HEK Cells.
- Purity
- >95 % as determined by SDS-PAGE and HPLC
- Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
- Biological Activity Comment
- The activity was tested by binding Human ACE2 in functional ELISA assay
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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- Comment
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Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
- Buffer
- Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 μm filtered PBS pH 7.4.
- Storage
- -20 °C
- Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Molecular Weight
- 140 kDa
- Gene ID
- 43740568
- UniProt
- P0DTC2
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