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SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha Host: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
Pubmed (1 reference)
Catalog No. ABIN6963742
$948.20
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  • Key Features
    • Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.1.7.
    • Contains del 69-70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H mutations characteristic for the SARS-CoV-2 alpha variant (B.1.1.7).
    Target See all SARS-CoV-2 Spike Proteins
    SARS-CoV-2 Spike
    Protein Characteristics
    B.1.1.7 - alpha
    Origin
    • 140
    • 23
    • 8
    • 8
    • 7
    • 6
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha
    Source
    • 134
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.
    Application
    ELISA, Ligand Binding Assay (LBA)
    Purpose
    This is the spike protein of the mutant strain B.1.1.7, also commonly known as the "UK / Great Britain mutant".
    Sequence
    Full-length sequence (aa 1 - 1273), del 69, del 70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
    furin cleavage site ""RRAR"" mutated to ""GSAG"", KV986PP
    C-terminal Rho1D4 tag fused with spacer ""GSSG"" to protein sequence
    Specificity
    Mutation that differ from canonical sequence of SPIKE protein: del 69-70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
    Characteristics
    "SARS CoV-2 full-length Spike B.1.1.7 Mutation"
    All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent.
    Purification
    affinity chromatography
    Purity
    > 98 % as determined by SDS-PAGE
  • Application Notes
    ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses
    Comment

    Further modifications:
    - furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
    - C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
    Size: 1286 amino acids (including Rho1D4 tag and linker)

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Avoid freeze-thaw cycles
  • Bhalla, Payam, Morelli, Sharma, Johnson, Thomson, Jolly, Canfarotta: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).

  • Target
    SARS-CoV-2 Spike
    Alternative Name
    SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)
    Synonyms
    E2, E2 glycoprotein precursor, Surface Glycoprotein, S
    Target Type
    Viral Protein
    Molecular Weight
    142114 Da
    UniProt
    P0DTC2
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