SARS-CoV-2 Spike Protein (B.1.351 - beta) (rho-1D4 tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Protein Characteristics
- B.1.351 - beta
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Origin
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.
- Application
- ELISA, Ligand Binding Assay (LBA)
- Purpose
- This is the spike protein of the mutant strain B.1.351, also commonly known as the "South Africa mutant".
- Specificity
- Mutation that differ from canonical sequence of SPIKE protein: del 144, K417N, E484K, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
- Characteristics
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"SARS CoV-2 full-length Spike B.1.351 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent. - Purification
- affinity chromatography
- Purity
- > 98 % as determined by SDS-PAGE
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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- Application Notes
- ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses
- Comment
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Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker) - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Avoid freeze-thaw cycles
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Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." in: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).
: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).
: "Durable protection against the SARS-CoV-2 Omicron variant is induced by an adjuvanted subunit vaccine. ..." in: Science translational medicine, Vol. 14, Issue 658, pp. eabq4130, (2022) (PubMed).
: "Measuring the Radius of Gyration and Intrinsic Flexibility of Viral Proteins in Buffer Solution Using Small-Angle X-ray Scattering." in: ACS measurement science au, Vol. 2, Issue 6, pp. 547-552, (2022) (PubMed).
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Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." in: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).
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- Target
- SARS-CoV-2 Spike
- Alternative Name
- SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Molecular Weight
- 142114 Da
- UniProt
- P0DTC2
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