SARS-CoV-2 Spike Protein (B.1.429 - epsilon) (rho-1D4 tag)

Catalog No. ABIN6972927

Product Details

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: B.1.429 - epsilon

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Epsilon

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.

Application: ELISA, Ligand Binding Assay (LBA)

Purpose: This is the spike protein of the mutant strain B.1.429 / CAL.20C also commonly known as the "California mutant".

Sequence: MFVFLVLLPL VSIQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SCMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YRYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PGSASSVASQ SIIAYTMSLG AENSVAYSNN SIAIPTNFTI SVTTEILPVS MTKTSVDCTM YICGDSTECS NLLLQYGSFC TQLNRALTGI AVEQDKNTQE VFAQVKQIYK TPPIKDFGGF NFSQILPDPS KPSKRSFIED LLFNKVTLAD AGFIKQYGDC LGDIAARDLI CAQKFNGLTV LPPLLTDEMI AQYTSALLAG TITSGWTFGA GAALQIPFAM QMAYRFNGIG VTQNVLYENQ KLIANQFNSA IGKIQDSLSS TASALGKLQD VVNQNAQALN TLVKQLSSNF GAISSVLNDI LSRLDPPEAE VQIDRLITGR LQSLQTYVTQ QLIRAAEIRA SANLAATKMS ECVLGQSKRV DFCGKGYHLM SFPQSAPHGV VFLHVTYVPA QEKNFTTAPA ICHDGKAHFP REGVFVSNGT HWFVTQRNFY EPQIITTDNT FVSGNCDVVI GIVNNTVYDP LQPELDSFKE ELDKYFKNHT SPDVDLGDIS GINASVVNIQ KEIYRLNEVA KNLNESLIDL QELGKYEQYI KWPWYIWLGF IAGLIAIVMV TIMLCCMTSC CSCLKGCCSC GSCCKFDEDD SEPVLKGVKL HYTGSSGTET SQVAPA

Specificity: Mutation that differ from "original" SPIKE protein: S13I, W152C, L452R, D1183Y

Characteristics: "SARS CoV-2 full-length Spike CAL.20C Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer these mutant SPIKE proteins.

Purification: Affnity Chromatography

Purity: >98% as determined by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG

Storage: -80 °C

Storage Comment: Store at -80°C. Avoid freeze-thaw cycles

Target Details

Target: SARS-CoV-2 Spike

Alternative Name: SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Molecular Weight: 142115.37 Da

UniProt: P0DTC2