SARS-CoV-2 Spike Protein (B.1.617.1 - kappa, Trimer) (His tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- B.1.617.1 - kappa, Trimer
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Origin
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with His tag.
- Purpose
- SARS-CoV-2 Spike Trimer (T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H), His Tag (MALS verified)
- Specificity
- SARS-CoV-2 Spike Trimer (T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H)
- Characteristics
- SARS-CoV-2 Spike Trimer, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (T95I, G142D, E154K, L452R, E484Q, D614G, P681R) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS
- Storage
- -20 °C
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Background
- It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 138.2 kDa
- NCBI Accession
- QHD43416
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