SARS-CoV-2 Spike Protein (BA.3 - Omicron, Trimer) (His tag)

Catalog No. ABIN7121370

Product Details

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: BA.3 - Omicron, Trimer

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (MALS verified)

Sequence: Val 16 - Pro 1213

Specificity: SARS-CoV-2 Spike Trimer (BA.3/Omicron)

Characteristics: SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (SPN-C5225) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (A67V,H69del,V70del,T95I,G142D,V143del,Y144del,Y145del,N211del,L212I,G339D,S371F,S373P,S375F,D405N,K417N,N440K,G446S,S477N,T478K,E484A,Q493R,Q498R,N501Y,Y505H,D614G,H655Y,N679K,P681H,N764K,D796Y,Q954H,N969K,R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.3, GISAID clade: GRA, Nextstrain clade: 21M). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Grade: MALS verified

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.7 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Products

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Background: Synonyms: Spike,S protein,Spike glycoprotein,S glycoprotein,
Description: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 137.7 kDa