The antibody for the detection of Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term) has now been successfully validated for immunohistochemistry. A signal was observed in human glioma tissue, while no signal could be detected in human brain tissue.The WNT gene family consists of structurally related genes encoding secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. » Read more
The antibody for the detection of Runt-Related Transcription Factor 3 (RUNX3) has now been successfully validated for immunofluorescence. A strong signal was observed in the human cellline A431 (epidermoid carcinoma), while no signal could be detected in the isotype staining and staining of HEK293 cells.A complex formed of a heterodimer of Runt-Related Transcription Factor 3 (RUNX3) and a beta subunit binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters. That can either activate or suppress transcription. RUNX3 can also interact with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. » Read more
The vial with the clear liquid, which Andreas Kessell has taken from the fridge, is several hundred dollars worth. Bedded on dry ice it goes on a journey to one of the 2000 customers from antibodies-online GmbH. The dealer from Aachen provides research institutes, university hospitals and pharmaceutical companies in 53 countries with antibodies. Scientists use them for development of therapies against cancer or rheumatism, but also for investigation of Alzheimer’s disease. With help of antibodies proteins can be identified or blocked or their absence can be proven.» Read more
The initiative kicked off in July 2013 but has only gained traction in the past few months, says Iorns. “We've done hundreds of tests so far, and we're planning to do 10,000 this year.”Successfully validated antibodies are awarded a green check mark “badge” for the vendor's web site. “We are trying to establish this as kind of a sign of quality,” says Stefan Pellenz, who runs the validation initiative at antibodies-online. But companies gain more from the process than just a logo, he adds; it is also a marketing tool, conveying to users that their reagent vendors take quality control seriously. Furthermore, the validating labs can work with the vendor (via Science Exchange) for troubleshooting. In one case, an ELISA kit shipped with a bad dilution buffer that was masking the target protein signal. Once the lab figured that out and reported it back to the vendor, the kit was updated subsequently passed validation “with flying colors,” Pellenz says. » Read more
The antibody for the detection of Prostate Specific Antigen (PSA) has now been successfully validated for flow cytometry. A strong signal was observed in the androgen-sensitive human prostate adenocarcinoma cellline LNCaP. The peak is clearly separated from the isotype stained probe.Prostate Specific Antigen (PSA) is a typical serine protease with Semenogelin-1 as its substrate. The cleavage of Semenogelin-1 liquefies the seiminal fluid. PSA is a tissue-specific biomarker and not a general marker for prostate cancer. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. Increased levels of PSA can be indicative of prostate cancer. » Read more
The antibody for the detection of Cadherin 5 (CDH5) has now been successfully validated for immunofluorescence. A clear signal of the calcium-depending adhesion molecule was achieved in murine liver tissue while murine brain tissue (known to be negative for cadherin 5) remained unstained.Cadherin 5 (also called CD144) is a vascular endothelial cadherin. This type of cadherins appears at the contact site of endothelial cells. They are functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, and additional they fundction also as a desmosome. » Read more
- Their purpose is to function as a barrier, protecting the body from mechanical damage, invading micro organisms and the loss of body fluids.
- Detection of epithelial cells has a central role in research on cancer diseases.
- A range of antibodies has been established for detection of individual epithelial cells.
- Some standard markers are: anti-epithelial membrane antigen (EMA) antibody and pan-cytokeratin antibody
- Starting Date: January 2014
- Employment Type: Permanent / Full Time
- Location of Position: Atlanta, GA
antibodies-online.com offers a wide range of immunology products covering research areas such as apoptosis, cytokines, cytokine receptors, chemokines, CD Markers and adhesion molecules.
Some example products are the following:
- Interleukin 4 Receptor (IL4R) ELISA Kit
- Interleukin 13 Receptor, alpha 2 (IL13RA2) ELISA Kit
- Intercellular Adhesion Molecule 3 (ICAM3) ELISA Kit
- Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) ELISA Kit
- Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
The above-mentioned ELISA kits are known for their batch-to-batch consistency as well as efficient delivery time.