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IRDye800 conjugated secondary antibodies

IRDye800 conjugated antibodies are specifically designed for protein detection methods that use near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications, like for example immunofluorescence microscopy with excitation/emission filters in the 780nm/820nm range. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Western blot detection levels for instance are in the picogram range. Some examples for secondary antibodies conjugated with IRDye800 are goat anti-chicken IgG, donkey anti-goat IgG, and rabbit anti-goat antibodies, as well as many more available on antibodies-online in the .

Find additional info about the theoretical background of secondary antibodies.