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An Introduction to ELISA

The Enzyme Linked Immunosorbent Assay (ELISA) is a class of popular analytical biochemistry assay designed to rapidly detect or quantify a specific analyte in a complex sample. Many different ELISA formats are presently used by life-science and biomedical researchers, often in the form of a pre-generated “assay kit.”

The antibodies-online resource “An Introduction to ELISA”, written by Ryan Robinson and Stefan Pellenz, explains ELISA basics in a quick, comprehensive format. Among the topics covered in this primer:

● How does the ELISA method work?

● Is an ELISA the right kind of assay for me?

● What is the difference between direct, indirect, sandwich, and competitive assay formats?

● Should I buy an ELISA kit, or build an assay from scratch?

Read more about ELISA in our resource section.