SARS-CoV-2 Spike Protein (B.1.621 - mu, RBD) (His tag)

Catalog No. ABIN7121335

Product Details

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: B.1.621 - mu, RBD

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Mu

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag (MALS verified)

Sequence: Arg 319 - Lys 537

Specificity: SARS-CoV-2 Spike RBD (R346K, E484K, N501Y)

Characteristics: SARS-CoV-2 Spike RBD, His Tag (SPD-C5221) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (R346K, E484K, N501Y). The mutations R346K, E484K, N501Y were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Grade: MALS verified

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Products

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Background: Synonyms: Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,
Description: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 26.5 kDa