When choosing an ELISA kit, most researchers demand an assay that is:
A sensitive assay is able to detect antigen at a very low titer in sample matrix (sometimes as low as pg/mL). A specific assay measures the target antigen only, and does not cross react with other common components inside normal sample matrices. A validated assay is accompanied by a sufficient quantity of data to confirm that both the sensitivity and specificity of the assay are consistent with the advertised specifications.
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